RNASEH2A Polyclonal antibody

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• RNASEH2A Polyclonal antibody (16132-1-AP)

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• KD/KO Validated

RNASEH2A Polyclonal Antibody for WB, IHC, ELISA

Cat No. 16132-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, ELISA

Synonyms

AGS4, Aicardi-Goutieres syndrome 4 protein, EC:3.1.26.4, JUNB, Ribonuclease H2 subunit A

• 

  WB analysis using 16132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 16132-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 16132-1-AP (RNASEH2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach using 16132-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 16132-1-AP (RNASEH2A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, human liver tissue, MCF-7 cells
• Positive IHC detected in: human stomach tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16132-1-AP targets RNASEH2A in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9118

  Product name: Recombinant human RNASEH2A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-299 aa of BC011748

  Sequence: MDLSELERDNTGRCRLSSPVPAVCRKEPCVLGVDEAGRGPVLGPMVYAICYCPLPRLADLEALKVADSKTLLESERERLFAKMEDTDFVGWALDVLSPNLISTSMLGRVKYNLNSLSHDTATGLIQYALDQGVNVTQVFVDTVGMPETYQARLQQSFPGIEVTVKAKADALYPVVSAASICAKVARDQAVKKWQFVEKLQDLDTDYGSGYPNDPKTKAWLKEHVEPVFGFPQFVRFSWRTAQTILEKEAEDVIWEDSASENQEGLRKITSYFLNEGSQARPRSSHRYFLERGLESATSL

• Full Name: ribonuclease H2, subunit A
• Calculated Molecular Weight: 299 aa, 33 kDa
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC011748
• Gene Symbol: RNASEH2A
• Gene ID (NCBI): 10535
• RRID: AB_2269729
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75792
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).

Publications

What published studies show

Anti-RNASEH2A (Cat# 16132-1-AP) has 8 total citations published in Oncogene, Cell Reports, Communications Biology, Oncology Reports, Cancer Research Communications, PLoS Genetics, Tumori, and bioRxiv. Research fields span cancer biology (prostate, breast, glioma, HCC), replication stress, genomic integrity, senescence-associated inflammation, Aicardi-Goutières syndrome, and cGAS-STING signaling. Applications include WB and IHC in human and mouse samples.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncogene; Human RNase H2 upregulation counteracts oncogene- and chemotherapy-induced replication stress; Authors - Rosanna J Wilkins
human	WB	Cell Rep; Pharmacological inhibition of neddylation impairs long interspersed element 1 retrotransposition; Authors - Yan Li ; KD Validated
human	WB	Commun Biol; RNaseH2A downregulation drives inflammatory gene expression via genomic DNA fragmentation in senescent and cancer cells; Authors - Sho Sugawara
		Oncol Rep; RNaseH2A is involved in human gliomagenesis through the regulation of cell proliferation and apoptosis.; Authors - Bin Dai
human	WB,IHC	Cancer Res Commun; Ribonuclease H2 Subunit A Preserves Genomic Integrity and Promotes Prostate Cancer Progression; Authors - Naoki Kimura ; KD Validated
human	WB	PLoS Genet; Aicardi-Goutières syndrome gene Rnaseh2c is a metastasis susceptibility gene in breast cancer.; Authors - Sarah K Deasy ; KD Validated