RNASEH2A Monoclonal antibody

RNASEH2A Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

WB

Conjugate

Unconjugated

CloneNo.

1A2E8

Cat no : 67976-1-Ig

Synonyms

AGS4, JUNB, Ribonuclease H2 subunit A, ribonuclease H2, subunit A, Ribonuclease HI large subunit, Ribonuclease HI subunit A, RNase H(35), RNase H2 subunit A, RNase HI large subunit, RNASEH2A, RNASEHI, RNHIA, RNHL

---

Validation Data Gallery

• 

  WB analysis using 67976-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67976-1-Ig (RNASEH2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: LNCaP cells, Hela cells, MCF-7 cells, HEK-293 cells, Jurkat cells, K-562 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67976-1-Ig targets RNASEH2A in WB applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: RNASEH2A fusion protein Ag9256
• Full Name: ribonuclease H2, subunit A
• Calculated Molecular Weight: 299 aa, 33 kDa
• GenBank Accession Number: BC011748
• Gene Symbol: RNASEH2A
• Gene ID (NCBI): 10535
• RRID: AB_2918725
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNASEH2A(Ribonuclease H2 subunit A) is also named as RNASEHI, RNHIA and belongs to the RNase HII family and Eukaryotic subfamily. It is involved in the removal of the initiator RNA primers of Okazaki fragments, an essential step during chromosomal DNA replication of the lagging strand(PMID:9482870). It is an endonuclease that specifically degrades the RNA of RNA:DNA hybrids. Defects in RNASEH2A are the cause of Aicardi-Goutieres syndrome type 4 (AGS4).