Tested Applications
| Positive WB detected in | THP-1 cells, RAW 264.7 cells |
| Positive IHC detected in | human tonsillitis tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
22577-1-AP targets RNASEL in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag17409 Product name: Recombinant human RNASEL protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 392-741 aa of BC115697 Sequence: KTFCEGSPRAQREVSCLQSSRENSHLVTFYGSESHRGHLFVCVTLCEQTLEACLDVHRGEDVENEEDEFAQNVLSSIFKAVQELHLSCGYTHQDLQPQNILIDSKKAAHLADFDKSIKWAGDPQEVKRDLEDLGRLVLYVVKKGSISFEELKAQSNEEVVQLSPDEETKDLIHRLFHPGEHVRDCLSDLLGHPFFWTWESRYRTLRNVGNESDIKTRKSESEILRLLQPGPSEHSKSFDKWTTKINECVMKKMNKFYEKRGNFYQNTVGDLLKFIRNLGEHIDEEKHKKMKLKIGDPSLYFQKTFPDLVIYVYTKLQNTEYRKHFPQTHSPNKPQCDGAGGASGLASPGC Predict reactive species |
| Full Name | ribonuclease L (2',5'-oligoisoadenylate synthetase-dependent) |
| Calculated Molecular Weight | 741 aa, 84 kDa |
| Observed Molecular Weight | 84 kDa; 73 kDa |
| GenBank Accession Number | BC115697 |
| Gene Symbol | RNASEL |
| Gene ID (NCBI) | 6041 |
| RRID | AB_2631036 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | Q05823 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNASEL, also named as RNS4, is an endoribonuclease that functions in the interferon (IFN) antiviral response. RNASEL mediated apoptosis is the result of a JNK-dependent stress-response pathway leading to cytochrome c release from mitochondria and caspase-dependent apoptosis. There're some isoforms with calculated MW 84 kDa and 73 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNASEL antibody 22577-1-AP | Download protocol |
| IHC protocol for RNASEL antibody 22577-1-AP | Download protocol |
| WB protocol for RNASEL antibody 22577-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-RNase L antibody (Cat# 22577-1-AP) has 11 total citations. Citations appear in Nature Cell Biology, Cardiovascular Diabetology, Advanced Science, Molecular Biomedicine, Apoptosis, Cell Reports, Virology Sinica, iScience, Hepatology Research, and Journal of the Formosan Medical Association. Research fields span type 1 and gestational diabetes, metabolic syndrome, RNA gene editing, cardiac ischemic injury, obesity, viral infections, interferon signaling, chronic hepatitis B, and diabetic wound healing.
| Species | Application | Title |
|---|---|---|
Nat Cell Biol Redox regulation of m6A methyltransferase METTL3 in β-cells controls the innate immune response in type 1 diabetes | ||
Cardiovasc Diabetol Human serum RNase-L level is inversely associated with metabolic syndrome and age. | ||
Adv Sci (Weinh) The CRISPR-Cas13a Gene-Editing System Induces Collateral Cleavage of RNA in Glioma Cells. | ||
Mol Biomed Activated oligoadenylate synthetase-ribonuclease L pathway promotes endothelial pyroptosis and impairs diabetic wound healing via thioredoxin-interacting protein m(6)A methylation.
| ||
Apoptosis The function of RNase L and its degradation mechanism in cardiac acute ischemic injury. | ||
Cell Rep PKR engages viral RNA and intron-retained host transcripts during poxvirus infection. |
