Tested Applications
| Positive WB detected in | PC-3 cells, Jurkat cells, human brain tissue |
| Positive IP detected in | PC-3 cells |
| Positive IHC detected in | mouse brain tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16936-1-AP targets RNF10 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10433 Product name: Recombinant human RNF10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 463-811 aa of BC016622 Sequence: LPEACDDLELADDNLKEGTICTESSQQEPITKSGFTRLSSSPCYYFYQAEDGQHMFLHPVNVRCLVREYGSLERSPEKISATVVEIAGYSMSEDVRQRHRYLSHLPLTCEFSICELALQPPVVSKETLEMFSDDIEKRKRQRQKKAREERRRERRIEIEENKKQGKYPEVHIPLENLQQFPAFNSYTCSSDSALGPTSTEGHGALSISPLSRSPGSHADFLLTPLSPTASQGSPSFCVGSLEEDSPFPSFAQMLRVGKAKADVWPKTAPKKDENSLVPPAPVDSDGESDNSDRVPVPSFQNSFSQAIEAAFMKLDTPATSDPLSEEKGGKKRKKQKQKLLFSTSVVHTK Predict reactive species |
| Full Name | ring finger protein 10 |
| Calculated Molecular Weight | 811 aa, 90 kDa |
| Observed Molecular Weight | 90 kDa |
| GenBank Accession Number | BC016622 |
| Gene Symbol | RNF10 |
| Gene ID (NCBI) | 9921 |
| RRID | AB_2181846 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8N5U6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IHC protocol for RNF10 antibody 16936-1-AP | Download protocol |
| IP protocol for RNF10 antibody 16936-1-AP | Download protocol |
| WB protocol for RNF10 antibody 16936-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RNF10 Polyclonal antibody (Cat# 16936-1-AP) has 12 total citations. These appear in journals including Nature Communications, Molecular Cell, Journal of Clinical Investigation, Cell Reports, Biomaterials, Acta Pharmacologica Sinica, Molecular Neurobiology, FEBS Letters, and eLife. Research fields span ribosome quality control and 40S ribosome modification, E3 ubiquitin ligase function, mitophagy and cardiac hypertrophy, neuronal morphology and NMDA receptor signaling, Myc-driven tumorigenesis, diabetic microenvironment modulation, and mitotic spindle integrity.
| Species | Application | Title |
|---|---|---|
Nat Commun E3 ubiquitin ligase RNF10 promotes dissociation of stalled ribosomes and responds to ribosomal subunit imbalance | ||
Mol Cell The integrated stress response regulates 18S nonfunctional rRNA decay in mammals
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Biomaterials Protean bio-heterojunction nanoparticle coating for dynamically modulating diabetic microenvironment. | ||
Acta Pharmacol Sin RNF10 deficiency in cardiomyocytes impairs mitophagy and drives pathological hypertrophy.
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Reviews
What customers say
The single review for this anti-RNF10 antibody reports a 5-star rating. The reviewer used it for Western blot on HCT116 cells at a 1:1000 dilution and described a clear, specific signal with reliable performance. Overall, the feedback is positive, highlighting the product's specificity and consistent results in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marta (Verified Customer) (07-22-2026) | I have used this anti-RNF10 antibody for Western blot in HCT116 cells, and it produced a clear, specific signal with reliable performance.
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