RNF10 Recombinant antibody, PBS Only

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  WB analysis of Jurkat using 86019-1-RR (same clone as 86019-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 86019-1-RR (same clone as 86019-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 86019-1-RR (same clone as 86019-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 86019-1-RR (RNF10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

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  IF Staining of K562 using 86019-1-RR (same clone as 86019-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed K562 using RNF10 antibody (86019-1-RR, Clone: 250438C11 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 86019-1-PBS in a different storage buffer formulation.

RNF10 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 86019-1-PBS

Clone No.250438C11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

E3 ubiquitin-protein ligase RNF10, EC:2.3.2.27, KIAA0262, RIE2, RING finger protein 10

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Product Information

86019-1-PBS targets RNF10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNF10 fusion protein Ag10433
• Full Name: ring finger protein 10
• Calculated Molecular Weight: 811 aa, 90 kDa
• Observed Molecular Weight: 90 kDa
• GenBank Accession Number: BC016622
• Gene Symbol: RNF10
• Gene ID (NCBI): 9921
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q8N5U6
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RING finger (C3HC4-type zinc finger) is a variant zinc finger motif present in a new family of proteins including transcription regulators. RNF10 (RING finger protein 10), a 90-kDa protein, contains a ring finger motif which is known to be involved in protein-protein interactions. RNF10 regulates myelin-associated glycoprotein (MAG) expression and is required for myelin formation, therefore, participates in the peripheral nerve development and Schawnn cell differentiation. RNF10 is also involved in activation of p21WAF1 promoter via its activity as transcriptional factor.