Tested Applications
| Positive WB detected in | Jurkat cells, MCF-7 cells |
| Positive IHC detected in | human ovary cancer tissue, human testis tissue, mouse testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | BGC-823 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
14338-1-AP targets RNF114 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag5649 Product name: Recombinant human RNF114 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-228 aa of BC066919 Sequence: MAAQQRDCGGAAQLAGPAAEADPLGRFTCPVCLEVYEKPVQVPCGHVFCSACLQECLKPKKPVCGVCRSALAPGVRAVELERQIESTETSCHGCRKNFFLSKIRSHVATCSKYQNYIMEGVKATIKDASLQPRNVPNRYTFPCPYCPEKNFDQEGLVEHCKLFHSTDTKSVVCPICASMPWGDPNYRSANFREHIQRRHRFSYDTFVDYDVDEEDMMNQVLQRSIIDQ Predict reactive species |
| Full Name | ring finger protein 114 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 26 kDa |
| GenBank Accession Number | BC066919 |
| Gene Symbol | RNF114 |
| Gene ID (NCBI) | 55905 |
| RRID | AB_3085435 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y508 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
RNF114, also known as ZNF228 or ZNF313, is a member of the zinc-binding protein family. RNF114 contains two C2H2 and Ring finger zinc-binding domains and a ubiquitin-interacting motif (UIM). RNF114 functions as an E3 ligase in the ubiquitin-proteasome system and plays an important role in basic cellular processes in different types of cells. RNF114 is expressed widely in many normal tissues, such as the heart, liver, and kidney. RNF114 has 2 isoforms with the molecular mass of 21 and 26 kDa. RNF114 is a novel determinant of psoriasis susceptibility, and its overexpression is a crucial indicator of epithelial inflammation (PMID: 34104941, 35069903, 37523804).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF114 antibody 14338-1-AP | Download protocol |
| IHC protocol for RNF114 antibody 14338-1-AP | Download protocol |
| WB protocol for RNF114 antibody 14338-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The anti-PARP7 antibody (Cat# 14338-1-AP) has 3 total citations. These appear in Advanced Science (IF 14.1), Cell Reports (IF 7.7), and bioRxiv. The research fields span male germ cell biology (heat shock granule disassembly) and metabolic regulation (NAD⁺ sensing by PARP7 in adipogenesis), with the latter studied in mouse models using Western blot and RNAi validation.
| Species | Application | Title |
|---|---|---|
Adv Sci (Weinh) BOULE is Essential for the Dynamic Disassembly of Heat Shock Granules in Male Germ Cells. | ||
Cell Rep NAD(+) sensing by PARP7 regulates the C/EBPβ-dependent transcription program during adipogenesis.
| ||
bioRxiv NAD + Sensing by PARP7 Regulates the C/EBPβ-Dependent Transcription Program in Adipose Tissue In Vivo
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