RNF125 Polyclonal antibody

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• RNF125 Polyclonal antibody (13290-1-AP)

RNF125 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13290-1-AP

Publications(6)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

ring finger protein 125, RNF125, TRAC 1, TRAC1

• 

  WB analysis of A2780 using 13290-1-AP

  A2780 cells were subjected to SDS PAGE followed by western blot with 13290-1-AP (RNF125 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 13290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13290-1-AP (RNF125 Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 13290-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 13290-1-AP (RNF125 Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A2780 cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

RNF125 expresses at a very low level in most cells and tissues.

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13290-1-AP targets RNF125 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3908

  Product name: Recombinant human RNF125 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-232 aa of BC012021

  Sequence: MGSVLSTDSGKSAPASATARALERRRDPELPVTSFDCAVCLEVLHQPVRTRCGHVFCRSCIATSLKNNKWTCPYCRAYLPSEGVPATDVAKRMKSEYKNCAECDIVLYLSEMRAHIRTCQKYIDKYGPLQELEETAARCVCPFCQRELYEDSLLDHCITHHRSERRPVFCPLCRLIPDENPSSFSGSLIRHLQVSHTLFYDDFIDFNIIEEALIRRVLDRSLLEYVNHSNTA

• Full Name: ring finger protein 125
• Calculated Molecular Weight: 232 aa, 26 kDa
• Observed Molecular Weight: 22-26 kDa
• GenBank Accession Number: BC012021
• Gene Symbol: RNF125
• Gene ID (NCBI): 54941
• RRID: AB_10597852
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96EQ8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncol Lett; RNF125‑mediated ubiquitination of MCM6 regulates the proliferation of human liver hepatocellular carcinoma cells; Authors - Xueyi Feng
human	WB	J Virol; PRRSV GP2a blocks the RLR signaling pathway by targeting RIG-I.; Authors - Yingjie Xiang
human	WB,IHC	Oncogene; RNF125 attenuates hepatocellular carcinoma progression by downregulating SRSF1-ERK pathway; Authors - Zhigang Feng ; KD Validated
human	WB,IP	Mol Cell Biochem; RNF125 promotes CD8 T cell-mediated anticancer immunity and suppresses the malignant phenotype of cervical cancer.; Authors - Di Yang
human	IHC	Oncol Rep; RNA‑binding protein MBNL1 regulates tumor growth, chemosensitivity and antitumor immunity in lung adenocarcinoma by controlling the expression of tumor suppressor RNF125; Authors - Yubo Yan
human	WB	Cell Rep; Type I interferon/STAT1 signaling regulates UBE2M-mediated antiviral innate immunity in a negative feedback manner; Authors - Xianghui Kong

Reviews

3.0 out of 5

1 verified customer reviews

What customers say

Automatically generated from the verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Jessica (Verified Customer) (01-31-2019)

Works well to detect overexpression of RNF125 in HEK293TCan barely detect RNF125 in HCT116 cells after exposure to 10h SeV.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T, HCT116