RNF126 Polyclonal antibody

• 

  WB analysis using 19835-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19835-1-AP (RNF126 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

RNF126 Polyclonal Antibody for WB, ELISA

Cat No. 19835-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

ring finger protein 126, RNF126

---

Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, LNCaP cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

19835-1-AP targets RNF126 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag13816

  Product name: Recombinant human RNF126 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-326 aa of BC001442

  Sequence: MAEASPHPGRYFCHCCSVEIVPRLPDYICPRCESGFIEELPEETRSTENGSAPSTAPTDQSRPPLEHVDQHLFTLPQGYGQFAFGIFDDSFEIPTFPPGAQADDGRDPESRRERDHPSRHRYGARQPRARLTTRRATGRHEGVPTLEGIIQQLVNGIITPATIPSLGPWGVLHSNPMDYAWGANGLDAIITQLLNQFENTGPPPADKEKIQALPTVPVTEEHVGSGLECPVCKDDYALGERVRQLPCNHLFHDGCIVPWLEQHDSCPVCRKSLTGQNTATNPPGLTGVSFSSSSSSSSSSSPSNENATWSPLGRPQPPRPLSNLTL

• Full Name: ring finger protein 126
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC001442
• Gene Symbol: RNF126
• Gene ID (NCBI): 55658
• RRID: AB_3085588
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BV68
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).