RNF126 Recombinant antibody

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  WB analysis using 84772-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84772-6-RR (RNF126 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 84772-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat pancreas using 84772-6-RR

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 84772-6-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 84772-6-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse ovary using 84772-6-RR

  Immunohistochemical analysis of paraffin-embedded mouse ovary tissue slide using 84772-6-RR (RNF126 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  FC experiment of Jurkat using 84772-6-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug RNF126 Recombinant antibody (84772-6-RR, Clone:242215A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  Affinity and Kinetic Characterization of 84772-6-RR

  Biolayer interferometry (BLl) kinetic assays of 84772-6-RR against Human RNF126 were performed. The affinity constant is below 1 pM.

RNF126 Uni-rAb^TM Recombinant Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 84772-6-RR

Clone No.242215A10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

242215A10, E3 ubiquitin-protein ligase RNF126, EC:2.3.2.27, RING finger protein 126

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Tested Applications

• Positive WB detected in: LNCaP cells, HEK-293 cells, HeLa cells, HepG2 cells, Jurkat cells, NIH/3T3 cells
• Positive IHC detected in: rat pancreas tissue, human ovary cancer tissue, human placenta tissue, mouse ovary tissue, mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84772-6-RR targets RNF126 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: RNF126 fusion protein Ag13816
• Full Name: ring finger protein 126
• Calculated Molecular Weight: 326 aa, 36 kDa
• Observed Molecular Weight: 36 kDa
• GenBank Accession Number: BC001442
• Gene Symbol: RNF126
• Gene ID (NCBI): 55658
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9BV68
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF126, also named as E3 ubiquitin-protein ligase RNF126, is a 326 amino acid protein, which is highly expressed in liver and testis. RNF126 (RING finger protein 126) contains one RING-type zinc finger domain and is known to interact with TRAF6 (a ubiquitin ligase) and BAT3 (an apoptotic regulator).