RNF128 Polyclonal antibody

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  WB analysis using 26015-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 26015-1-AP (RNF128 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 26015-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26015-1-AP (RNF128 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 26015-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 26015-1-AP (RNF128 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RNF128 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 26015-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

RING-type E3 ubiquitin transferase RNF128, RING finger protein 128, GRAIL, EC:2.3.2.27, E3 ubiquitin-protein ligase RNF128

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

26015-1-AP targets RNF128 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF128 fusion protein Ag22834
• Full Name: ring finger protein 128
• Calculated Molecular Weight: 428 aa, 47 kDa
• Observed Molecular Weight: 40-47 kDa, 66-70 kDa
• GenBank Accession Number: BC063404
• Gene Symbol: RNF128
• Gene ID (NCBI): 79589
• RRID: AB_2880336
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TEB7
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Stem Cell Res Ther; Requirement of splicing factor hnRNP A2B1 for tumorigenesis of melanoma stem cells.; Authors - Mengqi Chu ; KD Validated