RNF138 Polyclonal antibody

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• RNF138 Polyclonal antibody (17039-1-AP)

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  WB analysis using 17039-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17039-1-AP (RNF138 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

RNF138 Polyclonal Antibody for WB, ELISA

Cat No. 17039-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

E3 ubiquitin-protein ligase RNF138, EC:2.3.2.27, hNARF, HSD 4, HSD-4

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Tested Applications

• Positive WB detected in: mouse liver tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17039-1-AP targets RNF138 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10558

  Product name: Recombinant human RNF138 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-245 aa of BC018107

  Sequence: MAEDLSAATSYTEDDFYCPVCQEVLKTPVRTTACQHVFCRKCFLTAMRESGAHCPLCRGNVTRRERACPERALDLENIMRKFSGSCRCCAKQIKFYRMRHHYKSCKKYQDEYGVSSIIPNFQISQDSVGNSNRSETSTSDNTETYQENTSSSGHPTFKCPLCQESNFTRQRLLDHCNSNHLFQIVPVTCPICVSLPWGDPSQITRNFVSHLNQRHQFDYGEFVNLQLDEETQYQTAVEESFQVNI

• Full Name: ring finger protein 138
• Calculated Molecular Weight: 245 aa, 28 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC018107
• Gene Symbol: RNF138
• Gene ID (NCBI): 51444
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8WVD3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF138 (ring finger protein 138) is an E3 ubiquitin ligase containing a RING finger domain, which mediates the ubiquitination and degradation of target proteins involved in diverse cellular processes. RNF138 is primarily localized in the nucleus and plays important roles in DNA damage repair, particularly in the homologous recombination (HR) pathway following double-strand DNA breaks. It regulates the recruitment and turnover of DNA repair factors through ubiquitin-dependent mechanisms, thereby contributing to genomic stability and cell survival under genotoxic stress. In addition to DNA repair, RNF138 has been implicated in the regulation of cell proliferation, cell cycle progression, and signal transduction. Dysregulation of RNF138 expression has been associated with multiple cancers, where it may function either as a tumor promoter or suppressor depending on cellular context.