RNF14 Polyclonal antibody

RNF14 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Cat no : 26368-1-AP

Synonyms

HRIHFB2038, HFB30, EC:2.3.2.31, E3 ubiquitin-protein ligase RNF14, ARA54

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Validation Data Gallery

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  WB analysis of HEK-293T using 26368-1-AP

  HEK-293T cells were subjected to SDS PAGE followed by western blot with 26368-1-AP (RNF14 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 26368-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 26368-1-AP (RNF14 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of COLO 320 using 26368-1-AP

  IP result of anti-RNF14 (IP:26368-1-AP, 4ug; Detection:26368-1-AP 1:600) with COLO 320 cells lysate 4000ug.

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  IHC staining of human colon cancer using 26368-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 26368-1-AP (RNF14 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HEK-293 using 26368-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using RNF14 antibody (26368-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Tested Applications

• Positive WB detected in: HEK-293T cells, COLO 320 cells
• Positive IP detected in: COLO 320 cells
• Positive IHC detected in: human colon cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26368-1-AP targets RNF14 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF14 fusion protein Ag23734
• Full Name: ring finger protein 14
• Observed Molecular Weight: 47-54 kDa
• GenBank Accession Number: BC126185
• Gene Symbol: RNF14
• Gene ID (NCBI): 9604
• RRID: AB_2880494
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.