RNF144A Polyclonal antibody

RNF144A Polyclonal Antibody for WB, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, hamster

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 26144-1-AP

Synonyms

KIAA0161, ring finger protein 144A, RNF144, RNF144A, UBCE7IP4, UbcM4 interacting protein 4

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Validation Data Gallery

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  WB analysis of MDA-MB-231 using 26144-1-AP

  MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 26144-1-AP (RNF144A antibody) at dilution of 1:1500 and incubated at room temperature for 1.5 hours.

• 

  IP experiment of CHO using 26144-1-AP

  IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3100ug.

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  IP experiment of CHO using 26144-1-AP

  IP result of anti-RNF144A (IP:26144-1-AP, 4ug; Detection:26144-1-AP 1:300) with CHO cells lysate 3080ug.

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Tested Applications

• Positive WB detected in: MDA-MB-231 cells
• Positive IP detected in: CHO cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

The immunogen of 26144-1-AP is RNF144A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, hamster
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF144A fusion protein Ag23508
• Full Name: ring finger protein 144A
• Observed Molecular Weight: 33 kDa
• GenBank Accession Number: BC050373
• Gene Symbol: RNF144A
• Gene ID (NCBI): 9781
• RRID: AB_2880402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	EMBO Rep; RNF144A promotes antiviral responses by modulating STING ubiquitination; Authors - Bo Yang