RNF144B/IBRDC2 Polyclonal antibody

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• RNF144B/IBRDC2 Polyclonal antibody (26306-1-AP)

RNF144B/IBRDC2 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 26306-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, CoIP, ELISA

Synonyms

IBRDC2, RNF144B, E3 ubiquitin-protein ligase RNF144B, EC:2.3.2.31, IBR domain-containing protein 2

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  WB analysis of HeLa using 26306-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HeLa using 26306-1-AP

  IP result of anti-RNF144B/IBRDC2 (IP:26306-1-AP, 4ug; Detection:26306-1-AP 1:300) with HeLa cells lysate 2400ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 26306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 26306-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody at dilution of 1:200 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 26306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 26306-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse adrenal gland using 26306-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse adrenal gland tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat adrenal gland using 26306-1-AP

  Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue slide using 26306-1-AP (RNF144B/IBRDC2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human kidney tissue, mouse kidney tissue, rat adrenal gland tissue, mouse adrenal gland tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

26306-1-AP targets RNF144B/IBRDC2 in WB, IHC, IP, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag23679

  Product name: Recombinant human RNF144B protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 123-187 aa of BC063311

  Sequence: VADCQTVCPVASSDPGQPVLVECPSCHLKFCSCCKDAWHAEVSCRDSQPIVLPTEHRALFGTDAE

• Full Name: ring finger protein 144B
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC063311
• Gene Symbol: RNF144B
• Gene ID (NCBI): 255488
• RRID: AB_2880473
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q7Z419
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RNF144B/IBRDC2 Polyclonal antibody (Cat# 26306-1-AP) has 1 total citation. The cited publication appeared in EMBO Reports (2024), investigating how RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation. The research field encompasses antiviral immunity and protein degradation pathways.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP,CoIP	EMBO Rep; RNF144B negatively regulates antiviral immunity by targeting MDA5 for autophagic degradation; Authors - Guoxiu Li