RNF145 Polyclonal antibody

• Featured Product
• KD/KO Validated

RNF145 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 24524-1-AP

Synonyms

ring finger protein 145, RNF145

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Validation Data Gallery

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  WB analysis using 24524-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 24524-1-AP (RNF145 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 24524-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 24524-1-AP (RNF145 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 24524-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24524-1-AP (RNF145 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 24524-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 24524-1-AP (RNF145 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HeLa cells, mouse kidney tissue, HepG2 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 2 publications below

Product Information

24524-1-AP targets RNF145 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF145 fusion protein Ag21463
• Full Name: ring finger protein 145
• Calculated Molecular Weight: 663 aa, 76 kDa
• Observed Molecular Weight: 76-79 kDa
• GenBank Accession Number: BC042684
• Gene Symbol: RNF145
• Gene ID (NCBI): 153830
• RRID: AB_2879589
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IP	Elife; The sterol-responsive RNF145 E3 ubiquitin ligase mediates the degradation of HMG-CoA reductase together with gp78 and Hrd1.; Authors - Sam A Menzies
human	WB,IP	EMBO J; Regulation of membrane fluidity by RNF145-triggered degradation of the lipid hydrolase ADIPOR2; Authors - Norbert Volkmar ; KO Validated
human	WB	World J Surg Oncol; Hypoxia-based classification and prognostic signature for clinical management of hepatocellular carcinoma; Authors - Ke Li