RNF160 Polyclonal antibody

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  WB analysis using 28452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28452-1-AP (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 28452-1-AP

  WB result of RNF160 antibody (28452-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF160 transfected HepG2 cells.

• 

  WB analysis using 28452-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 28452-1-AP (RNF160 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse colon using 28452-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 28452-1-AP (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 28452-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using RNF160 antibody (28452-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

• Featured Product
• KD/KO Validated

RNF160 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 28452-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27, HSPC087

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Tested Applications

• Positive WB detected in: BxPC-3 cells, HepG2 cells, HeLa cells, mouse pancreas tissue, rat pancreas tissue
• Positive IHC detected in: mouse colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 7 publications below

Product Information

28452-1-AP targets RNF160 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29467

  Product name: Recombinant human RNF160 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC140790

  Sequence: MGGKNKQRTKGNLRPSNSGRAAELLAKEQGTVPGFIGFGTSQSDLGYVPAIQGAEEIDSLVDSDFRMVLRKLSKKDVTTKLKAMQEFGTMCTERDTETVKGVLPYWPRIFCKISLDHDRRVREATQQAFEKLILKVKKQLAPYLKSLMGYWLMAQCDTYTPAAFAAKDAFEAAFPPSKQPEAIAFCKDEITSVLQDHLIKETPDTLSDPQTVPEEEREAKFYRVVTCSLLALKRLLCLLPDNELDSLEEKFKSLLSQNKFWKYGKHSVPQIRSAYFELVSALCQRIPQLMKEEASKVSPS

• Full Name: ring finger protein 160
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC140790
• Gene Symbol: RNF160
• Gene ID (NCBI): 26046
• RRID: AB_2881144
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O94822
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human		Nat Commun; Stalled translation by mitochondrial stress upregulates a CNOT4-ZNF598 ribosomal quality control pathway important for tissue homeostasis; Authors - Ji Geng
human	WB	Cell Rep; NEMF-mediated Listerin-independent mitochondrial translational surveillance by E3 ligase Pirh2 and mitochondrial protease ClpXP; Authors - Liang Lv
human	WB	Acta Neuropathol Commun; Inefficient quality control of ribosome stalling during APP synthesis generates CAT-tailed species that precipitate hallmarks of Alzheimer's disease; Authors - Suman Rimal
human		Cell Rep; The E3 ubiquitin ligase FBXL6 controls the quality of newly synthesized mitochondrial ribosomal proteins; Authors - Julie Lavie
human	WB	bioRxiv; Ribosomal quality control factors inhibit repeat-associated non-AUG translation from GC-rich repeats; Authors - Yi-Ju Tseng
human	WB	bioRxiv; Nascent Chain Ubiquitination is Uncoupled from Degradation to Enable Protein Maturation; Authors - Xia Li