RNF160 Monoclonal antibody

RNF160 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, rat, mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D2A2

Cat no : 68610-1-Ig

Synonyms

C21orf10, C21orf98, FLJ11053, KIAA0714, ring finger protein 160, RNF160, Zinc finger protein 294, ZNF294

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Validation Data Gallery

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  WB analysis using 68610-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68610-1-Ig (RNF160 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: A549 cells, MCF-7 cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells, U2OS cells, HSC-T6 cells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

68610-1-Ig targets RNF160 in WB, ELISA applications and shows reactivity with Human, rat, mouse samples.

• Tested Reactivity: Human, rat, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RNF160 fusion protein Ag29471
• Full Name: ring finger protein 160
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC140790
• Gene Symbol: RNF160
• Gene ID (NCBI): 26046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).