RNF160 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82926-1-RR (same clone as 82926-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82926-1-RR (RNF160 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 82926-1-RR (same clone as 82926-1-PBS)

  WB result of RNF160 antibody (82926-1-RR; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RNF160 transfected HepG2 cells. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse colon using 82926-1-RR (same clone as 82926-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 82926-1-RR (RNF160 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  FC experiment of U2OS using 82926-1-RR (same clone as 82926-1-PBS)

  1x10^6 U2OS cells were intracellularly stained with 0.25 ug RNF160 Recombinant antibody (82926-1-RR, Clone:230075H2) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 82926-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82926-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82926-1-RR against Human ADAMDEC1 were performed. The affinity constant is below 1 pM.

• Featured Product
• KD/KO Validated

RNF160 Uni-rAb^® Recombinant Antibody for WB, IHC, FC (Intra), Indirect ELISA

Cat No. 82926-1-PBS

Clone No.230075H2

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), Indirect ELISA

Synonyms

230075H2, C21orf10, C21orf98, E3 ubiquitin-protein ligase listerin, EC:2.3.2.27

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Product Information

82926-1-PBS targets RNF160 in WB, IHC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag29467

  Product name: Recombinant human RNF160 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-300 aa of BC140790

  Sequence: MGGKNKQRTKGNLRPSNSGRAAELLAKEQGTVPGFIGFGTSQSDLGYVPAIQGAEEIDSLVDSDFRMVLRKLSKKDVTTKLKAMQEFGTMCTERDTETVKGVLPYWPRIFCKISLDHDRRVREATQQAFEKLILKVKKQLAPYLKSLMGYWLMAQCDTYTPAAFAAKDAFEAAFPPSKQPEAIAFCKDEITSVLQDHLIKETPDTLSDPQTVPEEEREAKFYRVVTCSLLALKRLLCLLPDNELDSLEEKFKSLLSQNKFWKYGKHSVPQIRSAYFELVSALCQRIPQLMKEEASKVSPS

• Full Name: ring finger protein 160
• Observed Molecular Weight: 180-200 kDa
• GenBank Accession Number: BC140790
• Gene Symbol: RNF160
• Gene ID (NCBI): 26046
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: O94822
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The E3 ubiquitin ligase, Ltn1 (also named RNF160), is a key regulator of RQC that targets substrates for ubiquitination (PMID: 35981909).