• Featured Product
  • KD/KO Validated

RNF2 Antibody

RNF2 Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16031-1-AP

Synonyms

BAP 1, BAP1, DING, HIP2 interacting protein 3, HIPI3, Protein DinG, RING finger protein 1B, ring finger protein 2, RING finger protein BAP 1, RING1B, RING2, RNF2



Tested Applications

Positive WB detected inK-562 cells, human brain tissue, NIH/3T3 cells, U-937 cells
Positive IP detected inK-562 cells
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

16031-1-AP targets RNF2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF2 fusion protein Ag8900
Full Name ring finger protein 2
Calculated molecular weight 336 aa, 38 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC012583
Gene symbol RNF2
Gene ID (NCBI) 6045
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RNF2 antibody 16031-1-APDownload protocol
IF protocol for RNF2 antibody 16031-1-APDownload protocol
IP protocol for RNF2 antibody 16031-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC,IF

Biochem Cell Biol

Knockdown of RNF2 enhances radiosensitivity of lung squamous cell carcinoma cells.

Authors - Jie Yang
  • KD Validated
humanWB

Wellcome Open Res

The role of the Xist 5' m6A region and RBM15 in X chromosome inactivation.

Authors - Heather Coker
humanIHC

Cancer Cell

The Polycomb Repressor Complex 1 Drives Double-Negative Prostate Cancer Metastasis by Coordinating Stemness and Immune Suppression.

Authors - Wenjing Su
  • KD Validated
humanWB

Cancer Cell

Coordinated regulation of polycomb group complexes through microRNAs in cancer.

Authors - Qi Cao
  • KD Validated