RNF212 Polyclonal antibody

• 

  WB analysis of human heart using 21178-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21178-1-AP (RNF212 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

RNF212 Polyclonal Antibody for WB, ELISA

Cat No. 21178-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

ring finger protein 212, RNF212, ZHP3

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Tested Applications

• Positive WB detected in: human heart tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

21178-1-AP targets RNF212 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag15457

  Product name: Recombinant human RNF212 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-192 aa of BC050356

  Sequence: MANWVFCNRCFQPPHRTSCFSLTNCGHVYCDACLGKGKKNECLICKAPCRTVLLSKHTDADIQAFFMSIDSLCKKYSRETSQILEFQEKHRKRLLAFYREKISRLEESLRKSVLQIEQLQSMRSSQQTAFSTIKSSVSTKPHGCLLQPHSSAPDRLESMEVDLSPSPIRKSEIAAGPARISMISPPQDGRMG

• Full Name: ring finger protein 212
• Calculated Molecular Weight: 297 aa, 33 kDa
• Observed Molecular Weight: 22 kDa
• GenBank Accession Number: BC050356
• Gene Symbol: RNF212
• Gene ID (NCBI): 285498
• RRID: AB_10863660
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q495C1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF212(RING finger protein 212)acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. It may be a SUMO E3 ligase that mediates the sumoylation of target proteins MSH4 and MSH5, leading to enhanced binding to recombination sites.

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

iru (Verified Customer) (07-14-2020)

I got a clean band but it is higher than the expected molecular weight.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Fallopian tube epithelial cells

RNF212 Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:21178-1-AP)