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RNF212 Polyclonal antibody

RNF212 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 21178-1-AP

Synonyms

ring finger protein 212, RNF212, ZHP3

Validation Data Gallery

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  • Western Blot (WB) analysis of human heart tissue using RNF212 Polyclonal antibody (21178-1-AP)

    WB analysis of human heart using 21178-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 21178-1-AP (RNF212 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inhuman heart tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21178-1-AP targets RNF212 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RNF212 fusion protein Ag15457
Full Name ring finger protein 212
Calculated Molecular Weight 297 aa, 33 kDa
Observed Molecular Weight 22 kDa
GenBank Accession NumberBC050356
Gene Symbol RNF212
Gene ID (NCBI) 285498
RRIDAB_10863660
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF212(RING finger protein 212)acts as a regulator of crossing-over during meiosis: required to couple chromosome synapsis to the formation of crossover-specific recombination complexes. It may be a SUMO E3 ligase that mediates the sumoylation of target proteins MSH4 and MSH5, leading to enhanced binding to recombination sites.

Protocols

Product Specific Protocols
WB protocol for RNF212 antibody 21178-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

iru (Verified Customer) (07-14-2020)

I got a clean band but it is higher than the expected molecular weight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Fallopian tube epithelial cells
RNF212 Antibody Western Blot validation (1:1000 dilution) in Fallopian tube epithelial cells (Cat no:21178-1-AP)