RNF213 Polyclonal antibody

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  WB analysis using 21028-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21028-1-AP (RNF213 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HUVEC using 21028-1-AP

  IP result of anti-RNF213 (IP:21028-1-AP, 4ug; Detection:21028-1-AP 1:300) with HUVEC cells lysate 910 ug.

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  IHC staining of human lymphoma using 21028-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

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  IHC staining of human lymphoma using 21028-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

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  IHC staining of human spleen using 21028-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

• 

  IHC staining of human spleen using 21028-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen slide using 21028-1-AP (RNF213 Antibody) at dilution of 1:50.

RNF213 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21028-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IP, ELISA

Synonyms

ring finger protein 213, Protein ALO17, KIAA1618, KIAA1554, C17orf27

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Tested Applications

• Positive WB detected in: HUVEC cells
• Positive IP detected in: HUVEC cells
• Positive IHC detected in: human lymphoma tissue, human spleen tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

21028-1-AP targets RNF213 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RNF213 fusion protein Ag15335
• Full Name: ring finger protein 213
• Calculated Molecular Weight: 3280 aa, 374 kDa
• GenBank Accession Number: BC032220
• Gene Symbol: RNF213
• Gene ID (NCBI): 57674
• RRID: AB_2878793
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q63HN8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF213 is a typical E3 ubiquitin ligase that can catalyze ubiquitination of both proteins and lipids, and which is involved in various processes, such as lipid metabolism, angiogenesis and cell-autonomous immunity (PMID: 21799892, 26126547, 26278786, 26766444, 30705059).RNF213 contains a C3HC4-type RING finger domain, which is a specialized type of Zn-finger that binds two atoms of zinc and is thought to be involved in mediating protein-protein interactions.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Thorac Cancer; RNF213 gene mutation in circulating tumor DNA detected by targeted next-generation sequencing in the assisted discrimination of early-stage lung cancer from pulmonary nodules.; Authors - Ning Jiang