RNF26 Polyclonal antibody

• 

  WB analysis of HepG2 using 16802-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16802-1-AP (RNF26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

RNF26 Polyclonal Antibody for WB, ELISA

Cat No. 16802-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

E3 ubiquitin-protein ligase RNF26, EC:2.3.2.27, RING finger protein 26

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16802-1-AP targets RNF26 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10285

  Product name: Recombinant human RNF26 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 239-433 aa of BC000058

  Sequence: AVTVTVLHPDFTLRLATQALSQLHARPSYHRLREDVMRLSRLALGSEAWRRVWSRSLQLASWPNRGGAPGAPQGDPMRVFSVRTRRQDTLPEAGRRSEAEEEEARTIRVTPVRGRERLNEEEPPGGQDPWKLLKEQEERKKCVICQDQSKTVLLLPCRHLCLCQACTEILMRHPVYHRNCPLCRRGILQTLNVYL

• Full Name: ring finger protein 26
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000058
• Gene Symbol: RNF26
• Gene ID (NCBI): 79102
• RRID: AB_2878317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BY78
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RNF26 is concentrated predominantly in the PN segment of the ER membrane, which corresponds with its ability to position all endosomal vesicles in the PN region of the cell. RNF26 resides in the perinuclear ER membrane, and this feature enables it to spatially restrict various endolysosomal species by attracting ubiquitin-binding vesicle adaptors through a ubiquitindependent ER MCS. RNF26 is an important E3 ubiquitin ligase that has been associated with poor prognosis in bladder cancer. And RNF26 is prevalent in normal human tissues but is upregulated in several human tumor cell lines (HL-60, HeLa S3 and SW480) (PMID: 33472082, 37519262).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Int J Biol Sci; The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC.; Authors - Wentao Liu ; KD Validated
human	WB,IHC,CoIP	Cell Death Dis; The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer.; Authors - Lu Yi ; KD Validated
human	WB,IP,IHC,IF	Br J Cancer; CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and increase the sensitivity of ccRCC to mTOR inhibitors; Authors - Xinlin Liu ; KD Validated
		Nat Commun; Small intestinal γδ T17 cells promote SAE through STING/C1q-induced microglial synaptic pruning in male mice; Authors - Yuming Wu
human	IHC	Oncogene; PTPRZ1 dephosphorylates and stabilizes RNF26 to reduce the efficacy of TKIs and PD-1 blockade in ccRCC; Authors - Yongkang Ma
human	WB	Am J Cancer Res; RNF26-mediated ubiquitination of TRIM21 promotes bladder cancer progression; Authors - Dongwei Yao ; KD Validated