RNF26 Polyclonal antibody

• 

  WB analysis of HepG2 using 16802-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16802-1-AP (RNF26 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• Featured Product
• KD/KO Validated

RNF26 Polyclonal Antibody for WB, ELISA

Cat No. 16802-1-AP

Publications(5)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, CoIP, ELISA

Synonyms

ring finger protein 26, RNF26

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Tested Applications

• Positive WB detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 4 publications below
• WB: See 4 publications below
• IHC: See 3 publications below
• IF: See 1 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Product Information

16802-1-AP targets RNF26 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10285

  Product name: Recombinant human RNF26 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 239-433 aa of BC000058

  Sequence: AVTVTVLHPDFTLRLATQALSQLHARPSYHRLREDVMRLSRLALGSEAWRRVWSRSLQLASWPNRGGAPGAPQGDPMRVFSVRTRRQDTLPEAGRRSEAEEEEARTIRVTPVRGRERLNEEEPPGGQDPWKLLKEQEERKKCVICQDQSKTVLLLPCRHLCLCQACTEILMRHPVYHRNCPLCRRGILQTLNVYL

• Full Name: ring finger protein 26
• Calculated Molecular Weight: 433 aa, 48 kDa
• Observed Molecular Weight: 48 kDa
• GenBank Accession Number: BC000058
• Gene Symbol: RNF26
• Gene ID (NCBI): 79102
• RRID: AB_2878317
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BY78
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IP	Int J Biol Sci; The RNF26/CBX7 axis modulates the TNF pathway to promote cell proliferation and regulate sensitivity to TKIs in ccRCC.; Authors - Wentao Liu ; KD Validated
human	WB,CoIP,IHC	Cell Death Dis; The FOXM1/RNF26/p57 axis regulates the cell cycle to promote the aggressiveness of bladder cancer.; Authors - Lu Yi ; KD Validated
human	WB,IP,IHC,IF	Br J Cancer; CDK4/6 inhibitors dephosphorylate RNF26 to stabilize TSC1 and increase the sensitivity of ccRCC to mTOR inhibitors; Authors - Xinlin Liu ; KD Validated
human	WB	Am J Cancer Res; RNF26-mediated ubiquitination of TRIM21 promotes bladder cancer progression; Authors - Dongwei Yao ; KD Validated
human	IHC	Oncogene; PTPRZ1 dephosphorylates and stabilizes RNF26 to reduce the efficacy of TKIs and PD-1 blockade in ccRCC; Authors - Yongkang Ma