Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, Jurkat cells, Raji cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
30797-1-AP targets RNF31/HOIP in WB, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag34505 Product name: Recombinant human RNF31 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 459-603 aa of BC017376 Sequence: RDCLFYLRDWTALRLQKLLQDNNVMFNTEPPAGARAVPGGGCRVIEQKEVPNGLRDEACGKETPAGYAGLCQAHYKEYLVSLINAHSLDPATLYEVEELETATERYLHVRPQPLAGEDPPAYQARLLQKLTEEVPLGQSIPRRRK Predict reactive species |
| Full Name | ring finger protein 31 |
| Calculated Molecular Weight | 1072 aa, 120 kDa |
| Observed Molecular Weight | 100 kDa |
| GenBank Accession Number | BC017376 |
| Gene Symbol | RNF31 |
| Gene ID (NCBI) | 55072 |
| RRID | AB_3086418 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96EP0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ring finger protein 31 (RNF31), also named HOIL-1-interacting protein (HOIP), ZIBRA, is an atypical E3 ubiquitin ligase, belongs to ring finger protein (RNF) family. It was first cloned from breast cancer cell line MCF7 and was identified as a classical component in linear ubiquitin assembly complex (LUBAC) to facilitate NFκB signaling transduction. It also plays important regulatory functions in controlling important oncogenic pathways in breast cancer, such as ERα and p53. RNF31 is highly expressed in muscle, heart, and testis. In cells, RNF31 mainly localizes to the cytoplasm. (PMID:27460922)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RNF31/HOIP antibody 30797-1-AP | Download protocol |
| WB protocol for RNF31/HOIP antibody 30797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Autophagy Advancing targeted protein degradation: pLIRTAC's role in glioma and CAR-T cell therapy.
| ||
J Clin Immunol Novel Compound Heterozygous Mutations in HOIP Result in Autoinflammation and Immunodeficiency. |
