RNF4 Recombinant monoclonal antibody, PBS Only (Capture)

• 

  IF Staining of HepG2 using 84770-1-RR (same clone as 84770-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RNF4 antibody (84770-1-RR, Clone: 242197A10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84770-1-PBS in a different storage buffer formulation.

• 

  FC experiment of MCF-7 using 84770-1-RR (same clone as 84770-1-PBS)

  1x10^6 MCF-7 cells were intracellularly stained with 0.25 ug RNF4 Recombinant antibody (84770-1-RR, Clone:242197A10) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 84770-1-PBS in a different storage buffer formulation.

• 

  Cytometric bead array standard curve of MP01553-1

  Cytometric bead array standard curve of MP01553-1, RNF4 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84770-1-PBS. Detection antibody: 84770-2-PBS. Standard: Ag12115. Range: 0.781-100 ng/mL.

• 

  Affinity and Kinetic Characterization of 84770-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84770-1-RR against Human RNF4 were performed. The affinity constant is 17.3 nM.

RNF4 Uni-rAb^® Recombinant Antibody for IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Cat No. 84770-1-PBS

Clone No.242197A10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, FC (Intra), Cytometric bead array, Indirect ELISA

Synonyms

242197A10, E3 ubiquitin ligase RNF4, E3 ubiquitin-protein ligase RNF4, EC:2.3.2.27, Protein SNURF

---

Product Information

84770-1-PBS targets RNF4 as part of a matched antibody pair:

MP01553-1: 84770-1-PBS capture and 84770-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag12115

  Product name: Recombinant human RNF4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-190 aa of BC031935

  Sequence: MSTRKRRGGAINSRQAQKRTREATSTPEISLEAEPIELVETAGDEIVDLTCESLEPVVVDLTHNDSVVIVDERRRPRRNARRLPQDHADSCVVSSDDEELSRDRDVYVTTHTPRNARDEGATGLRPSGTVSCPICMDGYSEIVQNGRLIVSTECGHVFCSQCLRDSLKNANTCPTCRKKINHKRYHPIYI

• Full Name: ring finger protein 4
• Calculated Molecular Weight: 190 aa, 21 kDa
• GenBank Accession Number: BC031935
• Gene Symbol: RNF4
• Gene ID (NCBI): 6047
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P78317
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RNF4 is a E3 ubiquitin-protein ligase that binds polysumoylated chains covalently attached to proteins and mediates 'Lys-6'-, 'Lys-11'-, 'Lys-48'- and 'Lys-63'-linked polyubiquitination of those substrates and their subsequent targeting to the proteasome for degradation. It regulates the degradation of several proteins including PML and PEA3[PMID:18408734]. It also participates in chromosome alignment and spindle assembly, and regulates the kinetochore CENPH-CENPI-CENPK complex by targeting polysumoylated CENPI to proteasomal degradation. In addition, RNF4 can regulates the cellular responses to hypoxia and heat shock through degradation of respectively EPAS1 and PARP1 [PMID:20026589]. Alternatively, it may also bind DNA/nucleosomes and have a more direct role in the regulation of transcription for instance enhancing basal transcription and steroid receptor-mediated transcriptional activation [PMID:11319220].