RNMTL1 Polyclonal antibody, PBS Only

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• RNMTL1 Polyclonal antibody, PBS Only (14707-1-PBS)

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  WB analysis of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 14707-1-AP (same clone as 14707-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 14707-1-AP (RNMTL1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

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  IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human liver using 14707-1-AP (same clone as 14707-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14707-1-AP (RNMTL1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 14707-1-AP (same clone as 14707-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using RNMTL1 antibody (14707-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 14707-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RNMTL1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 14707-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

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Product Information

14707-1-PBS targets RNMTL1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6399

  Product name: Recombinant human RNMTL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-420 aa of BC050614

  Sequence: MAALVRPSRFVVRPLLQVVQAWDLDARRWVRALRRSPVKVVFPSGEVVEQKRAPGKQPRKAPSEASAQEQREKQPLEESASRAPSTWEESGLRYDKAYPGDRRLSSVMTIVKSRPFREKQGKILLEGRRLISDALKAGAVPKMFFFSRLEYLKELPVDKLKGVSLIKVKFEDIKDWSDLVTPQGVMGIFAKPDHVKMTYPKTQLQHSLPLLLICDNLRDPGNLGTILRSAAGAGCSKVLLTKGCVDAWEPKVLRAGMGAHFRMPIINNLEWETVPNYLPPDTRVYVADNCGLYAQAEMSNKASDHGWVCDQRVMKFHKYEEEEDVETGASQDWLPHVEVQSYDSDWTEAPAAVVIGGETYGVSLESLQLAESTGGKRLLIPVVPGVDSLNSAMAASILLFEGKRQLRGRAEDLSRDRSYH

• Full Name: RNA methyltransferase like 1
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 47 kDa
• GenBank Accession Number: BC050614
• Gene Symbol: RNMTL1
• Gene ID (NCBI): 55178
• RRID: AB_2181563
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9HC36
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

The RNA methyltransferase like 1 gene (RNMTL1) is one of thirteen newly discovered genes within a 116 Kb segment of the chromosome 17p13.3, which suffers from a high frequent loss of heterozygosity in human hepatocellular carcinoma. Its amino acid sequence was identified a high conserved region with methylases from various microorganisms, suggesting to have function to transfer methyl group to rRNA molecule [PMID:12296377].