RNPC3 Polyclonal antibody

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  WB analysis of 3T3-L1 using 25820-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 25820-1-AP (RNPC3 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 25820-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 25820-1-AP (RNPC3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse spleen using 25820-1-AP

  IP result of anti-RNPC3 (IP:25820-1-AP, 4ug; Detection:25820-1-AP 1:300) with mouse spleen tissue lysate 4000ug.

• Featured Product
• KD/KO Validated

RNPC3 Polyclonal Antibody for WB, IP, ELISA

Cat No. 25820-1-AP

Publications(3)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

KIAA183, KIAA1839, RBM40, RNA binding motif protein 40, RNA binding protein 40, RNP, RNPC3, U11/U12 65K, U11/U12 snRNP 65 kDa protein

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Tested Applications

• Positive WB detected in: 3T3-L1 cells, mouse spleen tissue
• Positive IP detected in: mouse spleen tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below

Product Information

25820-1-AP targets RNPC3 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22957

  Product name: Recombinant human RNPC3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 169-517 aa of BC010697

  Sequence: LANIVNALASVPKFYVQVLHLMNKMNLPTPFGPITARPPMYEDYMPLHAPLPPTSPQPPEEPPLPDEDEELSSEESEYESTDDEDRQRMNKLMELANLQPKRPKTIKQRHVRKKRKIKDMLNTPLCPSHSSLHPVLLPSDVFDQPQPVGNKRIEFHISTDMPAAFKKDLEKEQNCEEKNHDLPATEVDASNIGFGKIFPKPNLDITEEIKEDSDEMPSECISRRELEKGRISREEMETLSVFRSYEPGEPNCRIYVKNLAKHVQEKDLKYIFGRYVDFSSETQRIMFDIRLMKEGRMKGQAFIGLPNEKAAAKALKEANGYVLFGKPMVVQFARSARPKQDPKEGKRKC

• Full Name: RNA-binding region (RNP1, RRM) containing 3
• Calculated Molecular Weight: 517 aa, 59 kDa
• Observed Molecular Weight: 62-65 kDa
• GenBank Accession Number: BC010697
• Gene Symbol: RNPC3
• Gene ID (NCBI): 55599
• RRID: AB_2880254
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96LT9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	PLoS Genet; Alternative exon definition events control the choice between nuclear retention and cytoplasmic export of U11/U12-65K mRNA.; Authors - Jens Verbeeren
mouse	WB	RNA; Early developmental arrest and impaired gastrointestinal homeostasis in U12-dependent splicing-defective Rnpc3-deficient mice.; Authors - Karen Doggett ; KO Validated
	WB	Adv Sci (Weinh); Minor Spliceosomal 65K/RNPC3 Interacts with ANKRD11 and Mediates HDAC3-Regulated Histone Deacetylation and Transcription; Authors - Chen-Hui Li