RNPEP Polyclonal antibody

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  WB analysis using 14764-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 14764-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 14764-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 14764-1-AP (RNPEP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of A549 using 14764-1-AP

  IP result of anti-RNPEP (IP:14764-1-AP, 4ug; Detection:14764-1-AP 1:500) with A549 cells lysate 2000ug.

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  IHC staining of human lung cancer using 14764-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 14764-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14764-1-AP (RNPEP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

RNPEP Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 14764-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

EC:3.4.11.6, AP-B, APB

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Tested Applications

• Positive WB detected in: HL-60 cells, HepG2 cells, A549 cells, HeLa cells, K-562 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: A549 cells
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below

Product Information

14764-1-AP targets RNPEP in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6526

  Product name: Recombinant human RNPEP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 300-650 aa of BC001064

  Sequence: MENPCLTFVTPCLLAGDRSLADVIIHEISHSWFGNLVTNANWGEFWLNEGFTMYAQRRISTILFGAAYTCLEAATGRALLRQHMDITGEENPLNKLRVKIEPGVDPDDTYNETPYEKGFCFVSYLAHLVGDQDQFDSFLKAYVHEFKFRSILADDFLDFYLEYFPELKKKRVDIIPGFEFDRWLNTPGWPPYLPDLSPGDSLMKPAEELAQLWAAEELDMKAIEAVAISPWKTYQLVYFLDKILQKSPLPPGNVKKLGDTYPSISNARNAELRLRWGQIVLKNDHQEDFWKVKEFLHNQGKQKYTLPLYHAMMGGSEVAQTLAKETFASTASQLHSNVVNYVQQIVAPKGS

• Full Name: arginyl aminopeptidase (aminopeptidase B)
• Calculated Molecular Weight: 72 kDa
• Observed Molecular Weight: 72 kDa
• GenBank Accession Number: BC001064
• Gene Symbol: RNPEP
• Gene ID (NCBI): 6051
• RRID: AB_2254005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H4A4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB	Mol Autism; RNA sequencing and proteomics approaches reveal novel deficits in the cortex of Mecp2-deficient mice, a model for Rett syndrome.; Authors - Natasha L Pacheco
human	WB	Cancer Sci; Tumor exosomal RNPEP promotes lung metastasis of liver cancer via inducing cancer-associated fibroblast activation; Authors - Yuankun Chen ; KD Validated