ROBO2 Polyclonal antibody

ROBO2 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 21635-1-AP

Synonyms

KIAA1568, ROBO2, Roundabout homolog 2, SAX3

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Validation Data Gallery

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  WB analysis using 21635-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21635-1-AP (ROBO2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below

Product Information

The immunogen of 21635-1-AP is ROBO2 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROBO2 fusion protein Ag16261
• Full Name: roundabout, axon guidance receptor, homolog 2 (Drosophila)
• Calculated Molecular Weight: 1378 aa, 151 kDa
• Observed Molecular Weight: 180 kDa
• GenBank Accession Number: BC146772
• Gene Symbol: ROBO2
• Gene ID (NCBI): 6092
• RRID: AB_2878893
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Ann Anat; Robo2 promotes osteoblast differentiation and mineralization through autophagy and is activated by parathyroid hormone induction; Authors - Ning Yuan ; KD Validated