ROD1 Polyclonal antibody

• Featured Product
• KD/KO Validated

ROD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 14027-1-AP

Synonyms

PTBP3, Regulator of differentiation 1, ROD1

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Validation Data Gallery

• 

  WB analysis of HL-60 using 14027-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14027-1-AP

  WB result of ROD1 antibody (14027-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-ROD1 transfected HeLa cells.

• 

  WB analysis using 14027-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14027-1-AP

  Various lysates subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse thymus using 14027-1-AP

  mouse thymus tissue were subjected to SDS PAGE followed by western blot with 14027-1-AP (ROD1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human lymphoma using 14027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human lymphoma using 14027-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 14027-1-AP (ROD1 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HL-60 cells, mouse thymus tissue, HeLa cells, Jurkat cells, rat thymus tissue
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

14027-1-AP targets ROD1 in WB, IHC, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROD1 fusion protein Ag5172
• Full Name: ROD1 regulator of differentiation 1 (S. pombe)
• Calculated Molecular Weight: 60 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC044585
• Gene Symbol: ROD1
• Gene ID (NCBI): 9991
• RRID: AB_2182321
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human,mouse	WB,CoIP	Cell Res; The RNA-binding protein ROD1/PTBP3 cotranscriptionally defines AID-loading sites to mediate antibody class switch in mammalian genomes.; Authors - Juan Chen ; KO Validated
human	WB	Cancer Cell Int; PTBP3 regulates proliferation of lung squamous cell carcinoma cells via CDC25A-mediated cell cycle progression.; Authors - Yingji Chen
human	WB,IHC	Cancer Cell Int; PTBP3 promotes migration of non-small cell lung cancer through regulating E-cadherin in EMT signaling pathway.; Authors - Qiong Wu
human	WB	Oncol Lett; Overexpression of ROD1 inhibits invasion of breast cancer cells by suppressing the translocation of β-catenin into the nucleus.; Authors - Ya Zhou
mouse	WB	Mol Cell; PTBP1-activated co-transcriptional splicing controls epigenetic status of pluripotent stem cells; Authors - Camilla Iannone

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Yaroslav (Verified Customer) (08-03-2022)

multiple bands detected at all tested dulitions (1:3000-1:10000)

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: mouse embryonic stem cells

ROD1 Antibody Western Blot validation (1:5000 dilution) in mouse embryonic stem cells (Cat no:14027-1-AP)