ROM1 Polyclonal antibody

ROM1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 21984-1-AP

Synonyms

ROM, ROM1, ROSP1, Tetraspanin 23, Tspan 23, TSPAN23

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Validation Data Gallery

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  WB analysis using 21984-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21984-1-AP (ROM1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21984-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 21984-1-AP (ROM1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 21984-1-AP

  Immunofluorescent analysis of HeLa cells using 21984-1-AP (ROM1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: mouse eye tissue, HeLa cells, rat eye tissue
• Positive IF detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 6 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

The immunogen of 21984-1-AP is ROM1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: ROM1 fusion protein Ag17049
• Full Name: retinal outer segment membrane protein 1
• Calculated Molecular Weight: 351 aa, 37 kDa
• Observed Molecular Weight: 37-45 kDa
• GenBank Accession Number: BC008100
• Gene Symbol: ROM1
• Gene ID (NCBI): 6094
• RRID: AB_2878961
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ROM1 (also known as TSPAN23) is a transmembrane protein present in photoreceptor outer segment disc membranes. ROM1 belongs to the tetraspanin superfamily, which are characterized by the presence of four conserved transmembrane regions. Tetraspanins have been involved in diverse processes such as cell activation and proliferation, adhesion and motility, differentiation, and cancer. ROM1 is required for rod photoreceptor viability and the regulation of disk morphogenesis (PMID: 10802659). It may function as an adhesion molecule involved in stabilization and compaction of outer segment disks.

Publications

Species	Application	Title
mouse	WB, IF	Proc Natl Acad Sci U S A; Accumulation of non-outer segment proteins in the outer segment underlies photoreceptor degeneration in Bardet-Biedl syndrome.; Authors - Poppy Datta
human	IF	Elife; Merging organoid and organ-on-a-chip technology to generate complex multi-layer tissue models in a human retina-on-a-chip platform.; Authors - Kevin Achberger
	IHC	Cell Mol Life Sci; A look into retinal organoids: methods, analytical techniques, and applications.; Authors - Tess A V Afanasyeva
mouse	WB,IF	J Biol Chem; The myosin-tail homology domain of centrosomal protein 290 is essential for protein confinement between the inner and outer segments in photoreceptors.; Authors - Poppy Datta
mouse	WB	PLoS One; Mouse models of human ocular disease for translational research.; Authors - Mark P Krebs
mouse	WB, IHC	PLoS One; Differential requirement of NPHP1 for compartmentalized protein localization during photoreceptor outer segment development and maintenance.; Authors - Poppy Datta