Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, T-47D cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25842-1-AP targets ROR2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22959 Product name: Recombinant human ROR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 781-943 aa of BC130522 Sequence: VSNARYVGPKQKAPPFPQPQFIPMKGQIRPMVPPPQLYIPVNGYQPVPAYGAYLPNFYPVQIPMQMAPQQVPPQMVPKPSSHHSGSGSTSTGYVTTAPSNTSMADRAALLSEGADDTQNAPEDGAQSTVQEAEEEEEGSVPETELLGDCDTLQVDEAQVQLEA Predict reactive species |
| Full Name | receptor tyrosine kinase-like orphan receptor 2 |
| Calculated Molecular Weight | 943 aa, 105 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC130522 |
| Gene Symbol | ROR2 |
| Gene ID (NCBI) | 4920 |
| RRID | AB_2918094 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01974 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tyrosine-protein kinase transmembrane receptor ROR2 belongs to the ROR-family of receptor tyrosine kinases (RTKs) (PMID: 12815588). ROR2 is a developmentally regulated protein that has significant expression and roles in a wide variety of tissues during early embryonic development. It was established that ROR2 plays a key role in skeletal and neural organogenesis, as well as in midgut development in the mouse, but then becomes repressed in adult tissues. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PMID: 25614331). Mutations in ROR2 gene are associated with autosomal recessive Robinow syndrome and autosomal dominant brachydactyly type B (PMID: 12815588).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ROR2 antibody 25842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Stephen (Verified Customer) (08-02-2022) | IP performed using in HeLa cells in RIPA buffer using 5ug of ror2 antibody per 1ug/ml of protein lysate. pulldown performed using magnetic protein A beads (NEB). western blot probed with another ror2 specific antibody raised in mouse ( MABS1152 )
 |
