Tested Applications
| Positive WB detected in | HeLa cells, HEK-293 cells, HepG2 cells, T-47D cells, K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25842-1-AP targets ROR2 in WB, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag22959 Product name: Recombinant human ROR2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 781-943 aa of BC130522 Sequence: VSNARYVGPKQKAPPFPQPQFIPMKGQIRPMVPPPQLYIPVNGYQPVPAYGAYLPNFYPVQIPMQMAPQQVPPQMVPKPSSHHSGSGSTSTGYVTTAPSNTSMADRAALLSEGADDTQNAPEDGAQSTVQEAEEEEEGSVPETELLGDCDTLQVDEAQVQLEA Predict reactive species |
| Full Name | receptor tyrosine kinase-like orphan receptor 2 |
| Calculated Molecular Weight | 943 aa, 105 kDa |
| Observed Molecular Weight | 105 kDa |
| GenBank Accession Number | BC130522 |
| Gene Symbol | ROR2 |
| Gene ID (NCBI) | 4920 |
| RRID | AB_2918094 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q01974 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tyrosine-protein kinase transmembrane receptor ROR2 belongs to the ROR-family of receptor tyrosine kinases (RTKs) (PMID: 12815588). ROR2 is a developmentally regulated protein that has significant expression and roles in a wide variety of tissues during early embryonic development. It was established that ROR2 plays a key role in skeletal and neural organogenesis, as well as in midgut development in the mouse, but then becomes repressed in adult tissues. It may act as a receptor for wnt ligand WNT5A which may result in the inhibition of WNT3A-mediated signaling (PMID: 25614331). Mutations in ROR2 gene are associated with autosomal recessive Robinow syndrome and autosomal dominant brachydactyly type B (PMID: 12815588).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for ROR2 antibody 25842-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
What customers say
The single review for 25842-1-AP is a 5-star rating for immunoprecipitation. The user performed IP in HeLa cells using RIPA buffer, with 5 µg of the ROR2 antibody per 1 µg/ml of protein lysate. Pulldown was carried out with magnetic Protein A beads, and the western blot was probed with a separate ROR2-specific mouse antibody. The protocol worked as expected, confirming the antibody's effectiveness for IP applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Stephen (Verified Customer) (08-02-2022) | IP performed using in HeLa cells in RIPA buffer using 5ug of ror2 antibody per 1ug/ml of protein lysate. pulldown performed using magnetic protein A beads (NEB). western blot probed with another ror2 specific antibody raised in mouse ( MABS1152 )
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