RORA Polyclonal antibody, PBS Only

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• RORA Polyclonal antibody, PBS Only (10616-1-PBS)

• 

  WB analysis using 10616-1-AP (same clone as 10616-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

• 

  WB analysis of PC-3 using 10616-1-AP (same clone as 10616-1-PBS)

  PC-3 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

• 

  WB analysis of K-562 using 10616-1-AP (same clone as 10616-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 10616-1-AP (same clone as 10616-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 10616-1-AP (same clone as 10616-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10616-1-AP (RORA antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10616-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

RORA Polyclonal Antibody for WB, ELISA

Cat No. 10616-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

NR1F1, Nuclear receptor ROR-alpha, Nuclear receptor RZR-alpha, RAR-related orphan receptor A, Retinoid-related orphan receptor-alpha

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Product Information

10616-1-PBS targets RORA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0944

  Product name: Recombinant human RORA protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 223-523 aa of BC008831

  Sequence: LDIQPSPDQSGLDINGIKPEPICDYTPASGFFPYCSFTNGETSPTVSMAELEHLAQNISKSHLETCQYLREELQQITWQTFLQEEIENYQNKQREVMWQLCAIKITEAIQYVVEFAKRIDGFMELCQNDQIVLLKAGSLEVVFIRMCRAFDSQNNTVYFDGKYASPDVFKSLGCEDFISFVFEFGKSLCSMHLTEDEIALFSAFVLMSADRSWLQEKVKIEKLQQKIQLALQHVLQKNHREDGILTKLICKVSTLRALCGRHTEKLMAFKAIYPDIVRLHFPPLYKELFTSEFEPAMQIDG

• Full Name: RAR-related orphan receptor A
• Calculated Molecular Weight: 59 kDa
• Observed Molecular Weight: 65-70 kDa
• GenBank Accession Number: BC008831
• Gene Symbol: RORA
• Gene ID (NCBI): 6095
• RRID: AB_2253844
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35398
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RORA is a steroid hormone receptor and has been implicated in the pathology of circadian rhythms, bone growth, angiogenesis, development of cones, cellular metabolism and a mediator in the immune and lipid metabolism pathways [PMID:21998696]. It binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence, which binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPAR gamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM and ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1 [PMID:11053433,14570920].