RORC Polyclonal antibody

RORC Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, ChIP, ELISA

Conjugate

Unconjugated

Publications

17

Cat no : 13205-1-AP

Synonyms

NR1F3, Nuclear receptor ROR gamma, Nuclear receptor RZR gamma, Nuclear receptor RZR-gamma, RAR related orphan receptor C, ROR gamma T, RORC, RORG, RORyT, RORγt, RZR GAMMA, RZRG, TOR

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Validation Data Gallery

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  WB analysis of HEK-293 using 13205-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13205-1-AP (RORC antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13205-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13205-1-AP (RORC antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, Jurkat cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 15 publications below
• IHC: See 2 publications below
• ChIP: See 1 publications below

Product Information

13205-1-AP targets RORC in WB, IHC, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, chicken
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RORC fusion protein Ag3961
• Full Name: RAR-related orphan receptor C
• Calculated molecular weight: 518 aa, 58 kDa
• Observed molecular weight: 58 kDa
• GenBank accession number: BC031554
• Gene symbol: RORC
• Gene ID (NCBI): 6097
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

The Nuclear receptors retinoic acid receptor-related orphan receptor gamma (RORγ or RORc, also known as NR1F3) was the third and most recent ROR group member to be discovered, following the prior disclosures of RORα (RORa or NR1F1) and RORβ (RORb or NR1F2) (PMID:24502334). Studies in mice suggest that the protein encoded by this gene may inhibit the expression of Fas ligand and IL2.RORC has 2 isoforms with the MW of 56 kDa and 58 kDa.

Publications

Species	Application	Title
human	WB,ChIP	Oncogene; Targeting the cholesterol-RORα/γ axis inhibits colorectal cancer progression through degrading c-myc; Authors - Ying-Nan Wang
rat	WB	Biomed Pharmacother; Yu Gan Long reduces rat liver fibrosis by blocking TGF-β1/Smad pathway and modulating the immunity.; Authors - Yu Xia
rat	WB	Phytomedicine; Extracts of Poria cocos improve functional dyspepsia via regulating brain-gut peptides, immunity and repairing of gastrointestinal mucosa; Authors - Yijun Tu
mouse	WB	Food Funct; Folic acid intervention changes liver Foxp3 methylation and ameliorates the damage caused by Th17/Treg imbalance after long-term alcohol exposure.; Authors - Huichao Zhao
mouse	WB	J Ethnopharmacol; The Chinese medicinal herb decoction QRZSLXF enhances anti-inflammatory effect in TNBS-induced colitis via balancing Th17/Tregs differentiation.; Authors - Man Zhang
mouse	WB	Int Immunopharmacol; Baicalin suppresses Th1 and Th17 responses and promotes Treg response to ameliorate sepsis-associated pancreatic injury via the RhoA-ROCK pathway.; Authors - Pingping Liu