Kazrin Polyclonal antibody

• Featured Product
• KD/KO Validated

Kazrin Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 11572-1-AP

Synonyms

KAZ, kazrin, KIAA1026, RP1 21O18.1, RP1-21O18.1

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Validation Data Gallery

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  WB analysis using 11572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 11572-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11572-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11572-1-AP (Kazrin antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11572-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 11572-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 11572-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 11572-1-AP (kazrin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11572-1-AP (kazrin antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of mouse skin using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skin using 11572-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skin tissue slide using 11572-1-AP (kazrin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse skin tissue, mouse heart tissue, human heart tissue, mouse brain tissue, rat heart tissue, rat skin tissue
• Positive IHC detected in: human colon cancer tissue, mouse skin tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IF: See 1 publications below

Product Information

11572-1-AP targets Kazrin in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Kazrin fusion protein Ag2152
• Full Name: kazrin
• Calculated Molecular Weight: 415 aa, 46 kDa
• Observed Molecular Weight: 46 kDa, 37 kDa
• GenBank Accession Number: BC035501
• Gene Symbol: Kazrin
• Gene ID (NCBI): 23254
• RRID: AB_833126
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Kazrin is an evolutionarily conserved protein that is upregulated during keratinocyte terminal differentiation. Kazrin localizes to desmosomes and binds the epidermal cornified envelope protein periplakin. Kazrin exists three isoforms:kazrin A (46.7 kDa), kazrin B (46 kDa), and kazrin C (37 kDa).

Publications

Species	Application	Title
mouse	WB,IF	J Invest Dermatol; Exons 5-15 of kazrin are dispensable for murine epidermal morphogenesis and homeostasis.; Authors - Chhatriwala Mariya K MK ; KD Validated