|Positive WB detected in
|HepG2 cells, Jurkat cells, HL-60 cells, MCF-7 cells, L02 cells
|Positive IP detected in
|Positive IHC detected in
|human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in
|Western Blot (WB)
|WB : 1:1000-1:4000
|IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
|IHC : 1:20-1:200
|IF : 1:50-1:500
|It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
|Sample-dependent, check data in validation data gallery
10847-1-AP targets MST4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|human, mouse, rat
|Host / Isotype
|Rabbit / IgG
|MST4 fusion protein Ag1302
|serine/threonine protein kinase MST4
|Calculated molecular weight
|16 kDa, 46 kDa
|Observed molecular weight
|GenBank accession number
|Gene ID (NCBI)
|Antigen affinity purification
|PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
|Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
|Product Specific Protocols
|WB protocol for MST4 antibody 10847-1-AP
|IHC protocol for MST4 antibody 10847-1-AP
|IF protocol for MST4 antibody 10847-1-AP
|IP protocol for MST4 antibody 10847-1-AP
|Click here to view our Standard Protocols
J Cell Sci
CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.
Int J Oncol
MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway.
Brain Res Bull
MST4 attenuates NLRP3 inflammasome-mediated neuroinflammation and affects the prognosis after intracerebral hemorrhage in mice.
Onco Targets Ther
MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression.
MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Guillaume (Verified Customer) (07-21-2022)
Antibody worked well, band present at 55kDa