• Featured Product
  • KD/KO Validated

MST4 Polyclonal antibody

MST4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10847-1-AP

Synonyms

MASK, MST 4, Mst3 and SOK1 related kinase, MST4, RP6 213H19.1, RP6-213H19.1, STE20 like kinase MST4, STK26



Tested Applications

Positive WB detected inHepG2 cells, Jurkat cells, HL-60 cells, MCF-7 cells, L02 cells
Positive IP detected inMCF-7 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

10847-1-AP targets MST4 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MST4 fusion protein Ag1302
Full Name serine/threonine protein kinase MST4
Calculated molecular weight 16 kDa, 46 kDa
Observed molecular weight 47-50 kDa
GenBank accession numberBC017213
Gene symbol RP6-213H19.1
Gene ID (NCBI) 51765
RRIDAB_513907
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MST4 antibody 10847-1-APDownload protocol
IHC protocol for MST4 antibody 10847-1-APDownload protocol
IF protocol for MST4 antibody 10847-1-APDownload protocol
IP protocol for MST4 antibody 10847-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

J Cell Sci

CLIC4 is a cytokinetic cleavage furrow protein that regulates cortical cytoskeleton stability during cell division.

Authors - Eric Peterman
  • KD Validated
humanWB,IHC,IF

Int J Oncol

MST4 promotes hepatocellular carcinoma epithelial-mesenchymal transition and metastasis via activation of the p-ERK pathway.

Authors - Zhen-Hai Lin
  • KD Validated
mouseWB,IF

Brain Res Bull

MST4 attenuates NLRP3 inflammasome-mediated neuroinflammation and affects the prognosis after intracerebral hemorrhage in mice.

Authors - Xiaodong Wu
humanIHC

Onco Targets Ther

MST4 Regulates Epithelial-Mesenchymal Transition of Choriocarcinoma by Mediating TGF-β1 Expression.

Authors - Hanxi Yu
mouseWB,IF

Behav Neurol

MST4 Kinase Inhibitor Hesperadin Attenuates Autophagy and Behavioral Disorder via the MST4/AKT Pathway in Intracerebral Hemorrhage Mice.

Authors - Xiaodong Wu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Guillaume (Verified Customer) (07-21-2022)

Antibody worked well, band present at 55kDa

  • Applications: Western Blot
  • Cell Tissue Type: Mice liver tumor/ Mice healthy liver
MST4 Antibody Western Blot validation ( dilution) in Mice liver tumor/ Mice healthy liver (Cat no:10847-1-AP)