RP9 Polyclonal antibody

• 

  WB analysis of HeLa using 16832-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16832-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16832-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 16832-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16832-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 16832-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

RP9 Polyclonal Antibody for WB, ELISA

Cat No. 16832-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PAP 1, PAP-1, Pim 1 associated protein, Pim-1-associated protein, Retinitis pigmentosa 9 protein

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

16832-1-AP targets RP9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10441

  Product name: Recombinant human RP9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-221 aa of BC025928

  Sequence: MSSRPGREDVGAAGARRPREPPEQELQRRREQKRRRHDAQQLQQLKHLESFYEKPPPGLIKEDETKPEDCIPDVPGNEHAREFLAHAPTKGLWMPLGKEVKVMQCWRCKRYGHRTGDKECPFFIKGNQKLEQFRVAHEDPMYDIIRDNKRHEKDVRIQQLKQLLEDSTSDEDRSSSSSSEGKEKHKKKKKKEKHKKRKKEKKKKKKRKHKSSKSNEGSDSE

• Full Name: retinitis pigmentosa 9 (autosomal dominant)
• Calculated Molecular Weight: 221 aa, 26 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC025928
• Gene Symbol: RP9
• Gene ID (NCBI): 6100
• RRID: AB_2878322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TA86
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.; Authors - Karl Bertram