RP9 Polyclonal antibody

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• RP9 Polyclonal antibody (16832-1-AP)

• KD/KO Validated

RP9 Polyclonal Antibody for WB, ELISA

Cat No. 16832-1-AP Copied!

Publications (1)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PAP 1, PAP-1, Pim 1 associated protein, Pim-1-associated protein, Retinitis pigmentosa 9 protein

• 

  WB analysis of HeLa using 16832-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16832-1-AP

  WB result of RP9 antibody (16832-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RP9 transfected HepG2 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 16832-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 16832-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16832-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 16832-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 16832-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 16832-1-AP (RP9 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells, mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16832-1-AP targets RP9 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10441

  Product name: Recombinant human RP9 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-221 aa of BC025928

  Sequence: MSSRPGREDVGAAGARRPREPPEQELQRRREQKRRRHDAQQLQQLKHLESFYEKPPPGLIKEDETKPEDCIPDVPGNEHAREFLAHAPTKGLWMPLGKEVKVMQCWRCKRYGHRTGDKECPFFIKGNQKLEQFRVAHEDPMYDIIRDNKRHEKDVRIQQLKQLLEDSTSDEDRSSSSSSEGKEKHKKKKKKEKHKKRKKEKKKKKKRKHKSSKSNEGSDSE

• Full Name: retinitis pigmentosa 9 (autosomal dominant)
• Calculated Molecular Weight: 221 aa, 26 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC025928
• Gene Symbol: RP9
• Gene ID (NCBI): 6100
• RRID: AB_2878322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8TA86
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RP9 antibody (Cat# 16832-1-AP) has 1 total citation. It appears in Molecular Cell (IF 16), in a study investigating metazoan-specific splicing factors in the human Step 1 spliceosome. The research field is RNA splicing and spliceosome biology. The citation validated the antibody for Western blot (WB) in human samples.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Mol Cell; Structural Insights into the Roles of Metazoan-Specific Splicing Factors in the Human Step 1 Spliceosome.; Authors - Karl Bertram

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product is highly positive, with a 5-star rating. The user performed a Western Blot on B cells at a 1:1000 dilution and reported clean signal, noting they used a blotting accelerator. Overall, the feedback highlights the antibody's strong performance and clear results in WB applications.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Annabel (Verified Customer) (12-18-2025)

Good antibody, used with blotting accelerator, clean signal

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: B Cells

RP9 Antibody Western Blot validation (1:1000 dilution) in B Cells (Cat no:16832-1-AP)