RPA1 Monoclonal antibody

RPA1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Rat and More (1)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

1G3F11

Cat no : 67973-1-Ig

Synonyms

HSSB, MST075, REPA1, Replication factor A protein 1, replication protein A1, 70kDa, RF A, RF A protein 1, RP A, RP A p70, RPA1, RPA70

---

Validation Data Gallery

• 

  WB analysis using 67973-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67973-1-Ig (RPA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

---

Tested Applications

• Positive WB detected in: HCT 116 cells, LNCaP cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

67973-1-Ig targets RPA1 in WB, IF, ELISA applications and shows reactivity with Human, Rat samples.

• Tested Reactivity: Human, Rat
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: RPA1 fusion protein Ag28239
• Full Name: replication protein A1, 70kDa
• Calculated Molecular Weight: 616 aa, 68 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC018126
• Gene Symbol: RPA1
• Gene ID (NCBI): 6117
• RRID: AB_2918723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IF	Cell Rep; HIV-1 exploits the Fanconi anemia pathway for viral DNA integration.; Authors - Shaozu Fu