|Positive WB detected in||HEK-293 cells, mouse brain tissue, HeLa cells, SH-SY5Y cells|
|Positive IP detected in||mouse kidney tissue|
|Positive IHC detected in||human gliomas tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HeLa cells|
|Western Blot (WB)||WB : 1:1000-1:6000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Immunofluorescence (IF)||IF : 1:50-1:500|
|Sample-dependent, check data in validation data gallery|
10412-1-AP targets RPA2 in WB, IP, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPA2 fusion protein Ag0690|
|Full Name||replication protein A2, 32kDa|
|Calculated molecular weight||29 kDa|
|Observed molecular weight||32 kDa|
|GenBank accession number||BC001630|
|Gene ID (NCBI)||6118|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.
|Product Specific Protocols|
|WB protocol for RPA2 antibody 10412-1-AP||Download protocol|
|IHC protocol for RPA2 antibody 10412-1-AP||Download protocol|
|IF protocol for RPA2 antibody 10412-1-AP||Download protocol|
|IP protocol for RPA2 antibody 10412-1-AP||Download protocol|
|Click here to view our Standard Protocols|
A methylation-phosphorylation switch determines Plk1 kinase activity and function in DNA damage repair.
Cell Death Dis
REV1 promotes lung tumorigenesis by activating the Rad18/SERTAD2 axis.
Cell Death Dis
Acquired temozolomide resistance in MGMTlow gliomas is associated with regulation of homologous recombination repair by ROCK2.
5-Azacytidine Enhances the Radiosensitivity of CNE2 and SUNE1 Cells In Vitro and In Vivo Possibly by Altering DNA Methylation.
In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.
J Genet Genomics
The ZFP541-KCTD19 complex is essential for pachytene progression by activating meiotic genes during mouse spermatogenesis.