RPA2 Polyclonal antibody

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  WB analysis using 10412-1-Ap

  Various lysates were subjected to SDS PAGE followed by western blot with 10412-1-Ap (RPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 10412-1-Ap

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10412-1-AP (RPA2 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 10412-1-Ap

  IP result of anti-RPA2 (IP:10412-1-Ap, 4ug; Detection:10412-1-Ap 1:1000) with mouse kidney tissue lysate 4000ug.

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  IHC staining of human gliomas using 10412-1-Ap

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10412-1-Ap (RPA2 antibody) at dilution of 1:100 (under 40x lens)..

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  IHC staining of human gliomas using 10412-1-Ap

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 10412-1-Ap (RPA2 antibody) at dilution of 1:100 (under 10x lens)..

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  IF Staining of A431 using 10412-1-Ap

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using RPA2 antibody (10412-1-Ap) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

RPA2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10412-1-AP

Publications(20)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ChIP, ELISA

Synonyms

RP A p34, RP A p32, RF-A protein 2, replication protein A2, 32kDa, Replication factor A protein 2

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse brain tissue, HeLa cells, SH-SY5Y cells
• Positive IP detected in: mouse kidney tissue
• Positive IHC detected in: human gliomas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 14 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Product Information

10412-1-AP targets RPA2 in WB, IHC, IF/ICC, IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPA2 fusion protein Ag0690
• Full Name: replication protein A2, 32kDa
• Calculated Molecular Weight: 29 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC001630
• Gene Symbol: RPA2
• Gene ID (NCBI): 6118
• RRID: AB_2269665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P15927
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPA2, a subunit of the heterotrimeric replication protein A (RPA) complex, is overexpressed in various cancers. RPA2 is a major target for upstream kinases, including CDKs and the three PIKKs (ATR, ataxia-telangiectasia mutated [ATM], and DNA-PK). RPA2 comprises multiple critical Ser/Tr residues that are phosphorylated in sequential order in response to genotoxic stress.

Publications

Species	Application	Title
human	WB	Sci Transl Med; Guanosine diphosphate-mannose suppresses homologous recombination repair and potentiates antitumor immunity in triple-negative breast cancer; Authors - Jia-Han Ding
human	WB	Mol Cell; A PARylation-phosphorylation cascade promotes TOPBP1 loading and RPA-RAD51 exchange in homologous recombination.; Authors - Jiao Zhao ; KD Validated
human	WB	Mol Cell; C1QBP Promotes Homologous Recombination by Stabilizing MRE11 and Controlling the Assembly and Activation of MRE11/RAD50/NBS1 Complex.; Authors - Yongtai Bai
human	WB	Nat Commun; Crosstalk between SUMOylation and ubiquitylation controls DNA end resection by maintaining MRE11 homeostasis on chromatin; Authors - Tao Zhang
human	WB,IF,IP	Cancer Lett; Disrupting PHF8-TOPBP1 connection elicits a breast tumor-specific vulnerability to chemotherapeutics.; Authors - Shuai Ma
human	WB	J Virol; Human Bocavirus 1 NP1 acts as an ssDNA-binding protein to help AAV2 DNA replication and cooperates with RPA to regulate AAV2 capsid expression; Authors - Yanqun Zhao