RPA3 Polyclonal antibody

RPA3 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC,ELISA

Conjugate

Unconjugated

Cat no : 10692-1-AP

Synonyms

REPA3, Replication factor A protein 3, replication protein A3, 14kDa, RF A protein 3, RP A p14, RPA14, RPA3



Tested Applications

Positive WB detected inK-562 cells, Jurkat cells, HeLa cells, HepG2 cells, A549 cells
Positive IHC detected inhuman lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:750-1:3000
Sample-dependent, check data in validation data gallery

Product Information

10692-1-AP targets RPA3 in WB, IHC,ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPA3 fusion protein Ag1065
Full Name replication protein A3, 14kDa
Calculated molecular weight 13 kDa
Observed molecular weight 14 kDa
GenBank accession numberBC005264
Gene symbol RPA3
Gene ID (NCBI) 6119
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for RPA3 antibody 10692-1-APDownload protocol
IHC protocol for RPA3 antibody 10692-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols