RPAIN Polyclonal antibody

RPAIN Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG


human, mouse





Cat no : 15220-1-AP


HRIP, RIP, RPA interacting protein, RPAIN

Tested Applications

Positive WB detected inA375 cells, mouse ovary tissue, A2780 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

15220-1-AP targets RPAIN in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPAIN fusion protein Ag7392
Full Name RPA interacting protein
Calculated molecular weight 25 kDa
Observed molecular weight 30-45 kDa
GenBank accession numberBC004451
Gene symbol RPAIN
Gene ID (NCBI) 84268
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.


Product Specific Protocols
WB protocol for RPAIN antibody 15220-1-APDownload protocol
IHC protocol for RPAIN antibody 15220-1-APDownload protocol
IP protocol for RPAIN antibody 15220-1-APDownload protocol
Standard Protocols
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