RPAIN Polyclonal antibody

RPAIN Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 15220-1-AP

Synonyms

HRIP, RIP, RPA interacting protein, RPAIN



Tested Applications

Positive WB detected inA375 cells, mouse ovary tissue, A2780 cells
Positive IP detected inA375 cells
Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:200-1:1000 for WB
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

15220-1-AP targets RPAIN in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPAIN fusion protein Ag7392
Full Name RPA interacting protein
Calculated molecular weight 25 kDa
Observed molecular weight 30-45 kDa
GenBank accession numberBC004451
Gene symbol RPAIN
Gene ID (NCBI) 84268
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

PAIN, also named as RIP, mediates the import of RPA complex into the nucleus, possibly via some interaction with importin beta. RPAIN has many isoforms with MW 25-27 kDa, 12 kDa and 16-19 kDa. The publication with PMID: 16135809 reported the MW 30 and 45 kDa.

Protocols

Product Specific Protocols
WB protocol for RPAIN antibody 15220-1-APDownload protocol
IHC protocol for RPAIN antibody 15220-1-APDownload protocol
IP protocol for RPAIN antibody 15220-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols