RPAP2 Polyclonal antibody

• Home
• Products
• Primary Antibodies
• RPAP2 Polyclonal antibody (17401-1-AP)

• Featured Product
• KD/KO Validated

RPAP2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 17401-1-AP Copied!

Publications (13)

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

RNA polymerase II-associated protein 2, EC:3.1.3.16, C1orf82

• 

  WB analysis using 17401-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17401-1-AP (RPAP2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 17401-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 17401-1-AP (RPAP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 17401-1-AP

  IP result of anti-RPAP2 (IP:17401-1-AP, 4ug; Detection:17401-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse cerebellum using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse brain using 17401-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17401-1-AP (RPAP2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HEK-293 using 17401-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using RPAP2 antibody (17401-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for RPAP2 in ?

Chat with Able

---

Tested Applications

• Positive WB detected in: Daudi cells, MCF-7 cells, HEK-293 cells, HeLa cells, MOLT-4 cells, THP-1 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human kidney tissue, mouse cerebellum tissue, mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

17401-1-AP targets RPAP2 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag11374

  Product name: Recombinant human RPAP2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-351 aa of BC039014

  Sequence: MADFAGPSSAGRKAGAPRCSRKAAGTKQTSTLKQEDASKRKAELEAAVRKKIEFERKALHIVEQLLEENITEEFLMECGRFITPAHYSDVVDERSIVKLCGYPLCQKKLGIVPKQKYKISTKTNKVYDITERKSFCSNFCYQASKFFEAQIPKTPVWVREEERHPDFQLLKEEQSGHSGEEVQLCSKAIKTSDIDNPSHFEKQYESSSSSTHSDSSSDNEQDFVSSILPGNRPNSTNIRPQLHQKSIMKKKAGHKANSKHKDKEQTVVDVTEQLGDCKLDSQEKDATCELPLQKVNTQSSSNSTLPERLKASENSESEYSRSEITLVGISKKSAEHFKRKFAKSNQVSRSV

• Full Name: RNA polymerase II associated protein 2
• Calculated Molecular Weight: 612 aa, 70 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC039014
• Gene Symbol: RPAP2
• Gene ID (NCBI): 79871
• RRID: AB_2180990
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q8IXW5
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPAP2 belongs to the RNA Pol II-associated protein (RPAP) family, a set of proteins involved in human Pol II machinery. Recently it has been reported that RPAP2 is required for efficient transcription of snRNA genes by Pol II. siRNA-mediated knockdown of RPAP2 caused defects in snRNA gene expression. (22137580)

Publications

What published studies show

The RPAP2 antibody (Cat# 17401-1-AP) has 13 citations across journals including Molecular Cell, Nature Structural & Molecular Biology, Nucleic Acids Research, Advanced Science, Bone Research, Cells, Histopathology, Transcription, Molecular Carcinogenesis, PLoS One, Gene, and Molecular Biomedicine. Research fields span RNA polymerase II transcription regulation, unfolded protein response and apoptosis, hepatocellular carcinoma, osteoporosis, myofibrillar myopathies, viral infection mechanisms, and cancer therapeutics.

Automatically generated from the 13 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human,mouse	WB,IF,CoIP	Bone Res; Super enhancers targeting ZBTB16 in osteogenesis protect against osteoporosis; Authors - Wenhui Yu ; KD Validated
human	WB	Mol Cell; Coordination between Two Branches of the Unfolded Protein Response Determines Apoptotic Cell Fate.; Authors - Tsun-Kai Chang ; KD Validated
human	WB	Mol Cell; Ser7 phosphorylation of the CTD recruits the RPAP2 Ser5 phosphatase to snRNA genes.; Authors - Egloff Sylvain S
human	WB	Nucleic Acids Res; Nuclear import of RNA polymerase II is coupled with nucleocytoplasmic shuttling of the RNA polymerase II-associated protein 2.; Authors - Forget Diane D
human	WB,IHC	Adv Sci (Weinh); The FBXW7-RPAP2 Axis Controls the Growth of Hepatocellular Carcinoma Cells and Determines the Fate of Liver Cell Differentiation; Authors - Danrui Cui
human	WB	Mol Biomed; RPRD1A drives lenvatinib resistance in hepatocellular carcinoma via the ITGA5-FAK signaling axis.; Authors - Xiaomeng Yao