RPAP3 Polyclonal antibody

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• RPAP3 Polyclonal antibody (23741-1-AP)

RPAP3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 23741-1-AP Copied!

Publications (5)

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Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

RNA polymerase II-associated protein 3

• 

  WB analysis of HEK-293 using 23741-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 23741-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 23741-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 23741-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 23741-1-AP (RPAP3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 23741-1-AP

  IP result of anti-RPAP3 (IP:23741-1-AP, 4ug; Detection:23741-1-AP 1:500) with HEK-293 cells lysate 2800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 23741-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human stomach cancer using 23741-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23741-1-AP (RPAP3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 23741-1-AP

  Immunofluorescent analysis of HeLa cells using 23741-1-AP (RPAP3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

23741-1-AP targets RPAP3 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20661

  Product name: Recombinant human RPAP3 protein

  Source: e coli.-derived, PET30a

  Tag: 6*His

  Domain: 1-360 aa of BC056415

  Sequence: MTSANKAIELQLQVKQNAEELQDFMRDLENWEKDIKQKDMELRRQNGVPEENLPPIRNGNFRKKKKGKAKESSKKTREENTKNRIKSYDYEAWAKLDVDRILDELDKDDSTHESLSQESESEEDGIHVDSQKALVLKEKGNKYFKQGKYDEAIDCYTKGMDADPYNPVLPTNRASAYFRLKKFAVAESDCNLAVALNRSYTKAYSRRGAARFALQKLEEAKKDYERVLELEPNNFEATNELRKISQALASKENSYPKEADIVIKSTEGERKQIEAQQNKQQAISEKDRGNGFFKEGKYERAIECYTRGIAADGANALLPANRAMAYLKIQKYEEAEKDCTQAILLDGSYSKAFARRGTAR

• Full Name: RNA polymerase II associated protein 3
• Calculated Molecular Weight: 76 kDa
• Observed Molecular Weight: 76 kDa
• GenBank Accession Number: BC056415
• Gene Symbol: RPAP3
• Gene ID (NCBI): 79657
• RRID: AB_2879313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• UNIPROT ID: Q9H6T3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPAP3 Polyclonal antibody (Cat# 23741-1-AP) has 5 total citations across journals including Oncogene, Cell Chemical Biology, Cell Reports, Biochemical and Biophysical Research Communications, and medRxiv. The associated research fields span gastric cancer (mTORC1/mRNA translation), lung cancer (PAQosome maturation, DNA replication, radioresistance), ciliogenesis (Akt signaling), oral squamous cell carcinoma (cell proliferation), and congenital disorders (snoRNP assembly, PEHO syndrome). All cited applications were Western blot in human samples.

Automatically generated from the 5 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Oncogene; PIWIL1 activates the R2TP-TELO2-mTORC1 axis independently of piRNA to promote TOP mRNA translation in gastric cancer; Authors - Tianqu Fan
human	WB	Cell Chem Biol; RUVBL1/RUVBL2 ATPase Activity Drives PAQosome Maturation, DNA Replication and Radioresistance in Lung Cancer.; Authors - Paul Yenerall
	WB	Cell Rep; DPCD is a regulator of R2TP in ciliogenesis initiation through Akt signaling; Authors - Yu-Qian Mao
human	WB	Biochem Biophys Res Commun; HEATR1, a novel interactor of Pontin/Reptin, stabilizes Pontin/Reptin and promotes cell proliferation of oral squamous cell carcinoma.; Authors - Akihiko Nakamura
human	WB	medRxiv; New ZNHIT3 Variants Disrupting snoRNP Assembly Cause Prenatal PEHO Syndrome with Isolated Hydrops; Authors - Md Lutfur Rahman