RPGRIP1L Polyclonal antibody

RPGRIP1L Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse, rat and More (2)

Applications

WB, IF, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 29778-1-AP

Synonyms

CORS3, DKFZp686C0668, FTM, JBTS7, KIAA1005, MKS5, Protein fantom, RPGRIP1 like, RPGRIP1 like protein, RPGRIP1L

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Validation Data Gallery

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  WB analysis using 29778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29778-1-AP (RPGRIP1L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 29778-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 29778-1-AP (RPGRIP1L antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 29778-1-AP

  Mouse testis tissue were subjected to SDS PAGE followed by western blot with 29778-1-AP (RPGRIP1L antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse testis tissue, HEK-293 cells, Jurkat cells, rat testis tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

29778-1-AP targets RPGRIP1L in WB, IF, ELISA applications and shows reactivity with Human, mouse, rat samples.

• Tested Reactivity: Human, mouse, rat
• Cited Reactivity: human, zebrafish
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPGRIP1L fusion protein Ag31168
• Full Name: RPGRIP1-like
• Calculated Molecular Weight: 151 kDa
• Observed Molecular Weight: 151 kDa
• GenBank Accession Number: NM_015272
• Gene Symbol: RPGRIP1L
• Gene ID (NCBI): 23322
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

RPGRIP1L, also named as FTM, KIAA1005, belongs to the RPGRIP1 family. It negatively regulates signaling through the G-protein coupled thromboxane A2 receptor. RPGRIP1L may be involved in mechanisms like programmed cell death, craniofacial development, patterning of the limbs, and formation of the left-right axis.(PMID:17558409) Defects in RPGRIP1L are the cause of Joubert syndrome type 7 (JBTS7). Defects in RPGRIP1L are the cause of Meckel syndrome type 5 (MKS5).

Publications

Species	Application	Title
human	IF	Elife; Centriolar satellites expedite mother centriole remodeling to promote ciliogenesis; Authors - Emma A Hall
human,zebrafish	WB	Dis Model Mech; Variable phenotypes and penetrance between and within different zebrafish ciliary transition zone mutants; Authors - Jun Wang