Tested Applications
| Positive IP detected in | HeLa cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:10-1:100 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16681-1-AP targets RPL10A in WB, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, xenopus |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10009 Product name: Recombinant human RPL10A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-217 aa of BC006791 Sequence: MSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKLVKKLAKKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKSTIKFQMKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRALYIKSTMGKPQRLY Predict reactive species |
| Full Name | ribosomal protein L10a |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC006791 |
| Gene Symbol | RPL10A |
| Gene ID (NCBI) | 4736 |
| RRID | AB_2181281 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P62906 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for RPL10A antibody 16681-1-AP | Download protocol |
| IP protocol for RPL10A antibody 16681-1-AP | Download protocol |
| WB protocol for RPL10A antibody 16681-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The RPL10A Polyclonal antibody (Cat# 16681-1-AP) has 14 citations. These appear in journals including Cell, Nature Communications, Neuron, Molecular Cell, Advanced Science, PNAS, Developmental Cell, Cell Reports, Translational Research, eLife, and CNS Neuroscience & Therapeutics. Research fields span axonal and ciliary local translation, mitochondrial function, circadian rhythms, Alzheimer's disease, colorectal cancer, intestinal epithelium homeostasis, T cell aging, testicular development, radial glial cilia, myeloma, and hearing loss. Applications cited include WB, IF, CoIP, and PLA across human, mouse, and Xenopus.
| Species | Application | Title |
|---|---|---|
Nat Commun Cilia locally synthesize proteins to sustain their ultrastructure and functions. | ||
Neuron Reconstructed cell-type-specific rhythms in human brain link Alzheimer's pathology, circadian stress, and ribosomal disruption. | ||
Neuron Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function. | ||
Mol Cell Chaperonin TRiC bridges radial spokes for folding locally translated proteins to sustain mammalian sperm flagellar motility. | ||
Adv Sci (Weinh) PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival |
Reviews
What customers say
The single review for this antibody rates it 5 out of 5 stars. The reviewer used it for Western Blot at a 1:2000 dilution and reported excellent performance, noting that it produced clean results with no non-specific bands when tested on HEK293T cell lysates. Overall, the feedback highlights the antibody's strong specificity and reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
S (Verified Customer) (11-22-2021) | this antibody is very good and didn't show non-specific bands in HEK293T cells.
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