RPL10A Polyclonal antibody

RPL10A Polyclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 16681-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IF/ICC, IP, CoIP, ELISA, PLA

NEDD6, NEDD 6, Large ribosomal subunit protein uL1, CSA-19, Csa 19

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHepG2 cells, HeLa cells, human brain tissue, human liver tissue
Positive IP detected inHeLa cells
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16681-1-AP targets RPL10A in WB, IF/ICC, IP, CoIP, ELISA, PLA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman, mouse, xenopus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10009

Product name: Recombinant human RPL10A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-217 aa of BC006791

Sequence: MSSKVSRDTLYEAVREVLHGNQRKRRKFLETVELQISLKNYDPQKDKRFSGTVRLKSTPRPKFSVCVLGDQQHCDEAKAVDIPHMDIEALKKLNKNKKLVKKLAKKYDAFLASESLIKQIPRILGPGLNKAGKFPSLLTHNENMVAKVDEVKSTIKFQMKKVLCLAVAVGHVKMTDDELVYNIHLAVNFLVSLLKKNWQNVRALYIKSTMGKPQRLY

Predict reactive species
Full Name ribosomal protein L10a
Calculated Molecular Weight 25 kDa
Observed Molecular Weight 25 kDa
GenBank Accession NumberBC006791
Gene Symbol RPL10A
Gene ID (NCBI) 4736
RRIDAB_2181281
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP62906
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for RPL10A antibody 16681-1-APDownload protocol
IF protocol for RPL10A antibody 16681-1-APDownload protocol
IP protocol for RPL10A antibody 16681-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
XenopusWB

Neuron

Axon-Axon Interactions Regulate Topographic Optic Tract Sorting via CYFIP2-Dependent WAVE Complex Function.

Authors - Jean-Michel Cioni
mouseIF

Nat Commun

Cilia locally synthesize proteins to sustain their ultrastructure and functions.

Authors - Kai Hao

Dev Cell

DDX20 is required for cell-cycle reentry of prospermatogonia and establishment of spermatogonial stem cell pool during testicular development in mice

Authors - Dingfeng Zou
humanFC

Transl Res

Ribosomal proteins as distinct "passengers" of microvesicles: new semantics in myeloma and mesenchymal stem cells' communication.

Authors - Mahmoud Dabbah
humanWB, PLA

Elife

Receptor-specific interactome as a hub for rapid cue-induced selective translation in axons.

Authors - Max Koppers
humanWB, CoIP

Adv Sci (Weinh)

Dual Inhibition of DKC1 and MEK1/2 Synergistically Restrains the Growth of Colorectal Cancer Cells.

Authors - Guangyan Kan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

S (Verified Customer) (11-22-2021)

this antibody is very good and didn't show non-specific bands in HEK293T cells.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
RPL10A Antibody Western Blot validation (1:2000 dilution) in (Cat no:16681-1-AP)
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