|Positive WB detected in||human liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue|
|Positive IHC detected in||human liver cancer tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||BxPC-3 cells|
|Western Blot (WB)||WB : 1:500-1:2000|
|Immunohistochemistry (IHC)||IHC : 1:50-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
16277-1-AP targets RPL11 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, mouse|
|Host / Isotype||Rabbit / IgG|
|Immunogen||RPL11 fusion protein Ag9356|
|Full Name||ribosomal protein L11|
|Calculated molecular weight||177 aa, 20 kDa|
|Observed molecular weight||20 kDa|
|GenBank accession number||BC018970|
|Gene ID (NCBI)||6135|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.
Oxid Med Cell Longev
BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis.
Knockdown of the Ribosomal Protein eL29 in Mammalian Cells Leads to Significant Changes in Gene Expression at the Transcription Level.
Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription.
DNA Repair (Amst)
Regulation of Actinomycin D induced upregulation of Mdm2 in H1299 cells.
Hum Mol Genet
FGFR2 Mutations in Bent Bone Dysplasia Syndrome Activate Nucleolar Stress and Perturb Cell Fate Determination.
Non-genotoxic activation of p53 through the RPL11-dependent ribosomal stress pathway.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Anita (Verified Customer) (12-22-2020)
Works for C. elegans at a protein concentration of 5 micrograms and above
Zehua (Verified Customer) (12-17-2019)
It works excellent for WB analysis. I strongly suggested this antibody.