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  • KD/KO Validated

RPL11 Polyclonal antibody

RPL11 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 16277-1-AP

Synonyms

60S ribosomal protein L11, CLL associated antigen KW 12, DBA7, GIG34, ribosomal protein L11, RPL11



Tested Applications

Positive WB detected inhuman liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue
Positive IF detected inBxPC-3 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16277-1-AP targets RPL11 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL11 fusion protein Ag9356
Full Name ribosomal protein L11
Calculated molecular weight 177 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC018970
Gene symbol RPL11
Gene ID (NCBI) 6135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.

Protocols

Product Specific Protocols
WB protocol for RPL11 antibody 16277-1-APDownload protocol
IF protocol for RPL11 antibody 16277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cells

Knockdown of the Ribosomal Protein eL29 in Mammalian Cells Leads to Significant Changes in Gene Expression at the Transcription Level.

Authors - Alexander V Gopanenko
humanWB,IF

Oxid Med Cell Longev

BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis.

Authors - Fangyuan Jia
  • KD Validated
humanIP

PLoS One

Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription.

Authors - Robin Assfalg
humanWB

DNA Repair (Amst)

Regulation of Actinomycin D induced upregulation of Mdm2 in H1299 cells.

Authors - Li Lianjie L
humanWB

Hum Mol Genet

FGFR2 Mutations in Bent Bone Dysplasia Syndrome Activate Nucleolar Stress and Perturb Cell Fate Determination.

Authors - Cynthia L Neben
humanWB

Carcinogenesis

Non-genotoxic activation of p53 through the RPL11-dependent ribosomal stress pathway.

Authors - Lucia Morgado-Palacin
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Anita (Verified Customer) (12-22-2020)

Works for C. elegans at a protein concentration of 5 micrograms and above

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C. elegans
RPL11 Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:16277-1-AP)
FH

Zehua (Verified Customer) (12-17-2019)

It works excellent for WB analysis. I strongly suggested this antibody.

  • Applications: Western Blot,Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cell lines
RPL11 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in Cell lines (Cat no:16277-1-AP)