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  • KD/KO Validated

RPL11 Polyclonal antibody

RPL11 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat





Cat no : 16277-1-AP


60S ribosomal protein L11, CLL associated antigen KW 12, DBA7, GIG34, ribosomal protein L11, RPL11

Tested Applications

Positive WB detected inhuman liver tissue, BxPC-3 cells, mouse lung tissue, mouse pancreas tissue, rat liver tissue
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inBxPC-3 cells

Recommended dilution

Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

16277-1-AP targets RPL11 in WB, IP, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen RPL11 fusion protein Ag9356
Full Name ribosomal protein L11
Calculated molecular weight 177 aa, 20 kDa
Observed molecular weight 20 kDa
GenBank accession numberBC018970
Gene symbol RPL11
Gene ID (NCBI) 6135
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Ribosomal protein L11 is one of 80 mammalian ribosome proteins. It can bind to 5S ribosomal RNA, for it's required for rRNA maturation and formation of the 60S ribosomal subunit. It can inhibit 40S or 60S ribosome biogenesis via mediated p53 induction. Once retained by PICT1 in the nucleolus, RPL11 involve in regulation of the MDM2-P53 pathway and promotion of tumor progression.


Product Specific Protocols
WB protocol for RPL11 antibody 16277-1-APDownload protocol
IHC protocol for RPL11 antibody 16277-1-APDownload protocol
IF protocol for RPL11 antibody 16277-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Oxid Med Cell Longev

BOP1 Knockdown Attenuates Neointimal Hyperplasia by Activating p53 and Inhibiting Nascent Protein Synthesis.

Authors - Fangyuan Jia
  • KD Validated


Knockdown of the Ribosomal Protein eL29 in Mammalian Cells Leads to Significant Changes in Gene Expression at the Transcription Level.

Authors - Alexander V Gopanenko

Int J Mol Sci

Deficiency of the Ribosomal Protein uL5 Leads to Significant Rearrangements of the Transcriptional and Translational Landscapes in Mammalian Cells.

Authors - Elena S Babaylova
  • KD Validated

PLoS One

Cellular sensitivity to UV-irradiation is mediated by RNA polymerase I transcription.

Authors - Robin Assfalg

Int J Oncol

Downregulated RRS1 inhibits invasion and metastasis of BT549 through RPL11‑c‑Myc‑SNAIL axis.

Authors - Runze Wang
  • KD Validated

DNA Repair (Amst)

Regulation of Actinomycin D induced upregulation of Mdm2 in H1299 cells.

Authors - Li Lianjie L


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Anita (Verified Customer) (12-22-2020)

Works for C. elegans at a protein concentration of 5 micrograms and above

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C. elegans
RPL11 Antibody Western Blot validation (1:1000 dilution) in C. elegans (Cat no:16277-1-AP)

Zehua (Verified Customer) (12-17-2019)

It works excellent for WB analysis. I strongly suggested this antibody.

  • Applications: Western Blot, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Cell lines
RPL11 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in Cell lines (Cat no:16277-1-AP)