RPL14 Recombinant monoclonal antibody, PBS Only

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  WB analysis using 82688-1-RR (same clone as 82688-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82688-1-RR (RPL14 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82688-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 82688-1-RR (same clone as 82688-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using RPL14 antibody (82688-1-RR, Clone: 2K22 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 82688-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82688-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82688-1-RR against Human RPL14 were performed. The affinity constant is below 1 pM.

RPL14 Uni-rAb^® Recombinant Antibody for WB, IF/ICC, Indirect ELISA

Cat No. 82688-1-PBS

Clone No.2K22

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

2K22, CAG ISL 7, CAG-ISL 7, CTG B33, hRL14

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Product Information

82688-1-PBS targets RPL14 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6976

  Product name: Recombinant human RPL14 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-216 aa of BC000606

  Sequence: MVFRRFVEVGRVAYVSFGPHAGKLVAIVDVIDQNRALVDGPCTQVRRQAMPFKCMQLTDFILKFPHSAHQKYVRQAWQKADINTKWAATRWAKKIEARERKAKMTDFDRFKVMKAKKMRNRIIKNEVKKLQKAALLKASPKKAPGTKGTAAAAAAAAAAAKVPAKKITAASKKAPAQKVPAQKATGQKAAPAPKAQKGQKAPAQKAPAPKASGKKA

• Full Name: ribosomal protein L14
• Calculated Molecular Weight: 23 kDa
• Observed Molecular Weight: 23-27 kDa
• GenBank Accession Number: BC000606
• Gene Symbol: RPL14
• Gene ID (NCBI): 9045
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P50914
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

RPL14 is a component of the large ribosomal subunit. The protein belongs to the L14E family of ribosomal proteins. It contains a basic region-leucine zipper (bZIP)-like domain.