RPL15 Polyclonal antibody

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• RPL15 Polyclonal antibody (16740-1-AP)

RPL15 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 16740-1-AP Copied!

Publications (7)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (1)

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

RPLY10, RPL10, ribosomal protein L15, Large ribosomal subunit protein eL15, EC45

• 

  WB analysis of COLO 320 using 16740-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of K-562 using 16740-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 16740-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse brain using 16740-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of SGC-7901 using 16740-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 16740-1-AP (RPL15 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human colon using 16740-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 16740-1-AP (RPL15 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 16740-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 16740-1-AP (RPL15 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: COLO 320 cells, HeLa cells, K-562 cells, mouse brain tissue, SGC-7901 cells
• Positive IHC detected in: human colon tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

16740-1-AP targets RPL15 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag9948

  Product name: Recombinant human RPL15 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-204 aa of BC070328

  Sequence: MGAYKYIQELWRKKQSDVMRFLLRVRCWQYRQLSALHRAPRPTRPDKARRLGYKAKQGYVIYRIRVRRGGRKRPVPKGATYGKPVHHGVNQLKFARSLQSVAEERAGRHCGALRVLNSYWVGEDSTYKFFEVILIDPFHKAIRRNPDTQWITKPVHKHREMRGLTSAGRKSRGLGKGHKFHHTIGGSRRAAWRRRNTLQLHRYR

• Full Name: ribosomal protein L15
• Calculated Molecular Weight: 204 aa, 24 kDa
• Observed Molecular Weight: 27 kDa
• GenBank Accession Number: BC070328
• Gene Symbol: RPL15
• Gene ID (NCBI): 6138
• RRID: AB_2301193
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P61313
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPL15 Polyclonal antibody (Cat# 16740-1-AP) has 7 citations spanning journals including Cell, Science Advances, Developmental Cell, Cell Reports, Frontiers in Immunology, and International Journal of Molecular Sciences. Research fields covered include ferroptosis in hematopoietic stem cells, tau-related neurodegeneration, autophagy during spermiogenesis, senescence and proteasome biology, ribosomal subunit maturation and translation, viral stress granule assembly, and epigenetic regulation in renal cell carcinoma.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell; Human hematopoietic stem cell vulnerability to ferroptosis; Authors - Jiawei Zhao
mouse	IF	Sci Adv; Neurons with granulovacuolar degeneration bodies are resilient to tau-induced protein synthesis impairment.; Authors - Jasper F. M. Smits
mouse	WB	Dev Cell; Autophagic elimination of ribosomes during spermiogenesis provides energy for flagellar motility.; Authors - Yuqing Lei
human	IF	Cell Rep; Senescent cells form nuclear foci that contain the 26S proteasome; Authors - Tomohiro Iriki
human	WB	Cell Rep; ZNF692 organizes a hub specialized in 40S ribosomal subunit maturation enhancing translation in rapidly proliferating cells; Authors - M Carmen Lafita-Navarro
pig	WB	Front Immunol; Foot-and-Mouth Disease Virus Counteracts on Internal Ribosome Entry Site Suppression by G3BP1 and Inhibits G3BP1-Mediated Stress Granule Assembly via Post-Translational Mechanisms.; Authors - Xu Ye

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

One reviewer (Sharvani Shintre) rated this product 5/5 after using it for Western Blot on mouse bone marrow-derived macrophages at a 1:2000 dilution. They reported a very clear single band at the expected 25 kDa molecular weight with no background bands, indicating strong specificity and sensitivity. Overall, the feedback is highly positive, highlighting clean results and reliable performance.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Sharvani (Verified Customer) (06-06-2025)

Very clear single band at the 25kDa with no background bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse bone marrow derived macrophages