RPL21 Polyclonal antibody

RPL21 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Cat no : 15226-1-AP

Synonyms

60S ribosomal protein L21, L21, ribosomal protein L21, RPL21

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Validation Data Gallery

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  WB analysis of HepG2 using 15226-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15226-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 15226-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 15226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 40x lens).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15226-1-AP targets RPL21 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RPL21 fusion protein Ag7444
• Full Name: ribosomal protein L21
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC001603
• Gene Symbol: RPL21
• Gene ID (NCBI): 6144
• RRID: AB_2181591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk