RPL21 Polyclonal antibody

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• RPL21 Polyclonal antibody (15226-1-AP)

RPL21 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15226-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ribosomal protein L21, Large ribosomal subunit protein eL21, L21, 60S ribosomal protein L21

• 

  WB analysis of HepG2 using 15226-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 15226-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 15226-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15226-1-AP (RPL21 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver using 15226-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15226-1-AP (RPL21 antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 15226-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using RPL21 antibody (15226-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15226-1-AP targets RPL21 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7444

  Product name: Recombinant human RPL21 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-160 aa of BC001603

  Sequence: MTNTKGKRRGTRYMFSRPFRKHGVVPLATYMRIYKKGDIVDIKGMGTVQKGMPHKCYHGKTGRVYNVTQHAVGIVVNKQVKGKILAKRINVRIEHIKHSKSRDSFLKRVKENDQKKKEAKEKGTWVQLKRQPAPPREAHFVRTNGKEPELLEPIPYEFMA

• Full Name: ribosomal protein L21
• Calculated Molecular Weight: 19 kDa
• Observed Molecular Weight: 20-25 kDa
• GenBank Accession Number: BC001603
• Gene Symbol: RPL21
• Gene ID (NCBI): 6144
• RRID: AB_2181591
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P46778
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPL21 Polyclonal antibody (Cat# 15226-1-AP) has 1 total citation. It was cited in iScience (IF 4.5) in a 2023 study investigating how TIAR and FMRP shape the pro-survival nascent proteome of leukemia cells within the bone marrow microenvironment. The cited application was Western Blot (WB) using human samples. The research field encompasses leukemia biology, bone marrow microenvironment interactions, and nascent proteome regulation.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment; Authors - Magdalena Wolczyk