RPL22 Polyclonal antibody

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• KD/KO Validated

RPL22 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 25002-1-AP Copied!

Publications (11)

Reviews (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF/ICC, IP, ELISA

Synonyms

60S ribosomal protein L22, EAP, EBER associated protein, EBER-associated protein, eL22

• 

  WB analysis of A431 using 25002-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 25002-1-AP

  WB result of RPL22 antibody (25002-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RPL22 transfected A431 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HepG2 using 25002-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 25002-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 25002-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 25002-1-AP (RPL22 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of A431 using 25002-1-AP

  IP result of anti-RPL22 (IP:25002-1-AP, 4ug; Detection:25002-1-AP 1:500) with A431 cells lysate 1920 ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 25002-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using RPL22 antibody (25002-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: A431 cells, HepG2 cells, HeLa cells, Jurkat cells
• Positive IP detected in: A431 cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25002-1-AP targets RPL22 in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag21851

  Product name: Recombinant human RPL22 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-128 aa of BC058887

  Sequence: MAPVKKLVVKGGKKKKQVLKFTLDCTHPVEDGIMDAANFEQFLQERIKVNGKAGNLGGGVVTIERSKSKITVTSEVPFSKRYLKYLTKKYLKKNNLRDWLRVVANSKESYELRYFQINQDEEEEEDED

• Full Name: ribosomal protein L22
• Calculated Molecular Weight: 15 kDa
• Observed Molecular Weight: 15-18 kDa
• GenBank Accession Number: BC058887
• Gene Symbol: RPL22
• Gene ID (NCBI): 6146
• RRID: AB_2879841
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P35268
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The RPL22 Polyclonal antibody (Cat# 25002-1-AP) has 11 citations spanning journals including Nature Cancer, Nature Communications, Molecular Cell, Science Advances, Cell Death Discovery, Cell Reports, Acta Neuropathologica Communications, Journal of Cell Biology, Molecular Neurobiology, Cell Cycle, and Carcinogenesis. Research fields covered include prostate cancer, stem cell biology, ribosome function, RNA modification, lung cancer, p53 regulation, Alzheimer's disease, RNA polymerase activity, neuroinflammation, cell cycle regulation, and colorectal cancer.

Automatically generated from the 11 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cancer; Targeting ADAR1 with a small molecule for the treatment of prostate cancer; Authors - Xiao Wang
human	WB	Nat Commun; A stem cell roadmap of ribosome heterogeneity reveals a function for RPL10A in mesoderm production; Authors - Naomi R Genuth
human	WB	Mol Cell; Early Scanning of Nascent Polypeptides inside the Ribosomal Tunnel by NAC.; Authors - Martin Gamerdinger
human	WB	Sci Adv; METTL3 regulates exocytosis independently of m(6)A.; Authors - Margalida Esteva-Socias
human	WB	Cell Death Discov; N6-methyladenine-mediated aberrant activation of the lncRNA SOX2OT-GLI1 loop promotes non-small-cell lung cancer stemness; Authors - Hongliang Dong
human	IF	Cell Rep; The ribosomal protein L22 binds the MDM4 pre-mRNA and promotes exon skipping to activate p53 upon nucleolar stress; Authors - Jennifer Jansen ; KD Validated

Reviews

5.0 out of 5

1 verified customer reviews

What customers say

The single review for this product is a 5-star rating from an Immunofluorescence application using colon cells at a 1:200 dilution. The reviewer described a standard protocol: cells grown on coverslips, fixed with 3.7% paraformaldehyde, permeabilized with 0.2% Triton X-100, blocked with 2% goat serum in PBS, then detected with the anti-PRL22 antibody followed by an Alexa Fluor 488-conjugated secondary antibody. The accompanying image supports the successful staining result.

Automatically generated from the 1 verified customer reviews below by Able AI.

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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Yuki (Verified Customer) (01-14-2021)

Cells were grown on sterile coverslips embedded in a 6-well plate. Cells were fixed with 3.7% paraformaldehyde and permeabilized with 0.2% Triton X-100. After blocking with 2% goat serum in PBS, the protein was detected using anti-PRL22 antibodies followed by reaction with Alexa Fluor488- conjugated secondary antibodies.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: colon cells

RPL22 Antibody Immunofluorescence validation (1:200 dilution) in colon cells (Cat no:25002-1-AP)