RPL34 Polyclonal antibody

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  WB analysis using 15179-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15179-1-AP (RPL34 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse pancreas using 15179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15179-1-AP (RPL34 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 15179-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15179-1-AP (RPL34 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 15179-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using RPL34 antibody (15179-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

RPL34 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15179-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ribosomal protein L34, Large ribosomal subunit protein eL34, 60S ribosomal protein L34

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells, PC-3 cells, mouse liver tissue
• Positive IHC detected in: mouse pancreas tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15179-1-AP targets RPL34 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag7100

  Product name: Recombinant human RPL34 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-117 aa of BC001773

  Sequence: MVQRLTYRRRLSYNTASNKTRLSRTPGNRIVYLYTKKVGKAPKSACGVCPGRLRGVRAVRPKVLMRLSKTKKHVSRAYGGSMCAKCVRDRIKRAFLIEEQKIVVKVLKAQAQSQKAK

• Full Name: ribosomal protein L34
• Calculated Molecular Weight: 13 kDa
• Observed Molecular Weight: 13 kDa
• GenBank Accession Number: BC001773
• Gene Symbol: RPL34
• Gene ID (NCBI): 6164
• RRID: AB_3085450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49207
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		J Cell Sci; Retromer/WASH dependent sorting of nutrient transporters requires a multivalent interaction network with ANKRD50.; Authors - Arunas Kvainickas